Niu E M, Crozat A, Catterall J F
The Population Council, Center for Biomedical Research, 1230 York Avenue, Box 273, 10021, New York, NY.
Endocrine. 1996 Feb;4(1):1-10. doi: 10.1007/BF02738868.
In mouse kidney, the kidney androgen-regulated protein (KAP) gene is regulated in a sex-dependent manner by a complex tissue- and cell-specific multihormonal system. KAP is also found in mouse uterus during the period surrounding birth. We describe here for the first time the existence of KAP in a species other than mouse. The rat cDNA sequence was determined and the derived peptide sequence displayed only 53% identity with murine KAP, although the genomic organization of the genes was identical. Expression of rat KAP was restricted to kidney and uterus, but was constitutive in the latter and drastically induced at parturition. The renal expression of the rat KAP gene was sexually dimorphic and regulated by physiological levels of steroid hormones. The effects of castration, hypophysectomy, thyroidectomy, and castration plus thyroidectomy on KAP mRNA levels in both kidney and uterus were determined. Constitutive expression of the protein was strictly dependent on thyroid hormone in female kidneys where it was modulated by estrogens and other ovarian factor(s). In the uterus, KAP mRNA was mainly under estrogen control. In males, expression of the KAP gene was under the dual regulation of thyroid hormone and androgens. Its complex regulation suggests a carefully delineated role for KAP in the kidney and uterus, but its physiological function remains unknown.
在小鼠肾脏中，肾脏雄激素调节蛋白（KAP）基因受一个复杂的组织和细胞特异性多激素系统以性别依赖的方式调控。在小鼠出生前后的子宫中也发现了KAP。我们首次在此描述了除小鼠外其他物种中KAP的存在。测定了大鼠的cDNA序列，尽管基因的基因组结构相同，但推导的肽序列与小鼠KAP仅显示53%的同一性。大鼠KAP的表达仅限于肾脏和子宫，但在子宫中是组成性表达，在分娩时急剧诱导。大鼠KAP基因的肾脏表达具有性别二态性，并受类固醇激素生理水平的调控。确定了去势、垂体切除、甲状腺切除以及去势加甲状腺切除对肾脏和子宫中KAP mRNA水平的影响。该蛋白的组成性表达在雌性肾脏中严格依赖于甲状腺激素，在雌性肾脏中它受雌激素和其他卵巢因子的调节。在子宫中，KAP mRNA主要受雌激素控制。在雄性中，KAP基因的表达受甲状腺激素和雄激素的双重调控。其复杂的调控表明KAP在肾脏和子宫中具有明确界定的作用，但其生理功能仍然未知。
