利用超高效液相色谱/电喷雾电离飞行时间质谱法检测法属波利尼西亚三种石松科植物中的生物碱及其抗胆碱酯酶活性。

Detection by UPLC/ESI-TOF-MS of alkaloids in three Lycopodiaceae species from French Polynesia and their anticholinesterase activity.

作者信息

Ho Raimana, Marsousi Niloufar, Eugster Philippe, Bianchini Jean-Pierre, Raharivelomanana Phila

机构信息

Laboratoire de Pharmacognosie et Phytochimie, Section des Sciences Pharmaceutiques, Université de Genève, Université de Lausanne, CH-1211 Genève 4, Switzerland.

出版信息

Nat Prod Commun. 2009 Oct;4(10):1349-52.

PMID:19911569

Abstract

Three Lycopodiaceae species from French Polynesia, Lycopodium venustulum C. Gaudichaud, Lycopodiella cernua (C. Linnaeus) R. E. Pichi Sermolli and Lycopodium henryanum E. D. Brown were investigated for their alkaloidal composition by UHPLC/ESI-TOF-MS. Ten alkaloids were identified, with lycopodine and lycodoline being the main constituents in the three species. The acetylcholinesterase-inhibitory activities of the three species are probably due to the occurrence of huperzine A, huperzine B, huperzine E, huperzinine and lycopodine.

摘要

对来自法属波利尼西亚的三种石松科植物，即秀丽石松（Lycopodium venustulum C. Gaudichaud）、垂穗石松（Lycopodiella cernua (C. Linnaeus) R. E. Pichi Sermolli）和亨利石松（Lycopodium henryanum E. D. Brown）进行了超高效液相色谱/电喷雾电离飞行时间质谱（UHPLC/ESI-TOF-MS）分析，以研究其生物碱成分。共鉴定出10种生物碱，石松碱和石松灵碱是这三种植物中的主要成分。这三种植物的乙酰胆碱酯酶抑制活性可能归因于石杉碱甲、石杉碱乙、石杉碱戊、石杉碱宁和石松碱的存在。