Xue Guanhua, Yu Lihua, Li Shentao, Shen Xuzhuang
Beijing Children's Hospital, Capital Medical University, Beijing, China.
FEMS Immunol Med Microbiol. 2010 Mar;58(2):202-10. doi: 10.1111/j.1574-695X.2009.00623.x. Epub 2009 Oct 12.
Sip and ScpB are highly conserved among strains of Group B Streptococcus (GBS). Thus, the two proteins are attractive antigens for inclusion in a vaccine against GBS. In this study, we constructed and expressed the two proteins, and investigated their specific mucosal immune responses against GBS induced by intranasal immunization with cholera toxin (CT) and CpG-oligodeoxynucleotides (CpG-ODNs). Intranasal immunization with different doses of recombinant Sip and ScpB all elicited specific antibodies in serum and vagina of mice. A combination of rSip and rScpB with either CT or CpG-ODN elicited specific antibodies in serum and vaginal samples. Th1 responses were enhanced by CpG and CT. Sera from the mice group intranasally immunized with rSip+CT, rScpB+CT, rSip+rScpB+CT, and rSip+rScpB+CpG also showed bactericidal activity compared with the serum of the control group. The current findings suggest that rSip and rScpB would be useful antigens as a vaccine component to induce protective immune responses against GBS, and CpG-ODN could be used as an effective mucosal adjuvant in inducing a good mucosal immune response. The use of an intranasal vaccine composed of different surface protein antigens is an attractive strategy for the development of a vaccine against GBS.
Sip和ScpB在B族链球菌（GBS）菌株中高度保守。因此，这两种蛋白质是用于GBS疫苗的有吸引力的抗原。在本研究中，我们构建并表达了这两种蛋白质，并研究了用霍乱毒素（CT）和CpG-寡脱氧核苷酸（CpG-ODN）经鼻免疫诱导的针对GBS的特异性黏膜免疫反应。用不同剂量的重组Sip和ScpB经鼻免疫均在小鼠血清和阴道中诱导产生了特异性抗体。rSip和rScpB与CT或CpG-ODN的组合在血清和阴道样本中诱导产生了特异性抗体。CpG和CT增强了Th1反应。与对照组血清相比，经鼻免疫rSip+CT、rScpB+CT、rSip+rScpB+CT和rSip+rScpB+CpG的小鼠组血清也显示出杀菌活性。目前的研究结果表明，rSip和rScpB作为疫苗成分可诱导针对GBS的保护性免疫反应，CpG-ODN可作为诱导良好黏膜免疫反应的有效黏膜佐剂。使用由不同表面蛋白抗原组成的鼻内疫苗是开发GBS疫苗的一种有吸引力的策略。
