Institut Pasteur-Cenci Bolognetti Foundation, Department of Experimental Medicine, University of Rome La Sapienza, Rome, Italy.
J Cell Mol Med. 2011 Jan;15(1):63-71. doi: 10.1111/j.1582-4934.2009.00968.x.
Experimental data suggest that cell-based therapies may be useful for cardiac regeneration following ischaemic heart disease. Bone marrow (BM) cells have been reported to contribute to tissue repair after myocardial infarction (MI) by a variety of humoural and cellular mechanisms. However, there is no direct evidence, so far, that BM cells can generate cardiac stem cells (CSCs). To investigate whether BM cells contribute to repopulate the Kit(+) CSCs pool, we transplanted BM cells from transgenic mice, expressing green fluorescent protein under the control of Kit regulatory elements, into wild-type irradiated recipients. Following haematological reconstitution and MI, CSCs were cultured from cardiac explants to generate 'cardiospheres', a microtissue normally originating in vitro from CSCs. These were all green fluorescent (i.e. BM derived) and contained cells capable of initiating differentiation into cells expressing the cardiac marker Nkx2.5. These findings indicate that, at least in conditions of local acute cardiac damage, BM cells can home into the heart and give rise to cells that share properties of resident Kit(+) CSCs.
实验数据表明，细胞疗法可能对缺血性心脏病后心脏再生有用。骨髓（BM）细胞已被报道通过多种体液和细胞机制在心肌梗死（MI）后促进组织修复。然而，到目前为止，还没有直接证据表明 BM 细胞可以产生心脏干细胞（CSC）。为了研究 BM 细胞是否有助于补充 Kit(+)CSC 池，我们将表达 Kit 调节元件控制下的绿色荧光蛋白的转基因小鼠的 BM 细胞移植到野生型照射受体中。在血液学重建和 MI 后，从心脏外植体培养 CSC 以生成“心脏球体”，这是一种通常来源于 CSC 的体外微组织。这些都是绿色荧光（即 BM 衍生），并包含能够起始分化为表达心脏标志物 Nkx2.5 的细胞的细胞。这些发现表明，至少在局部急性心脏损伤的情况下，BM 细胞可以归巢到心脏，并产生具有驻留 Kit(+)CSC 特性的细胞。
