State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071, China.
BMC Nephrol. 2012 Sep 10;13:105. doi: 10.1186/1471-2369-13-105.
Bone marrow (BM) stem cells have been reported to contribute to tissue repair after kidney injury model. However, there is no direct evidence so far that BM cells can trans-differentiate into renal stem cells.
To investigate whether BM stem cells contribute to repopulate the renal stem cell pool, we transplanted BM cells from transgenic mice, expressing enhanced green fluorescent protein (EGFP) into wild-type irradiated recipients. Following hematological reconstitution and ischemia-reperfusion (I/R), Sca-1 and c-Kit positive renal stem cells in kidney were evaluated by immunostaining and flow cytometry analysis. Moreover, granulocyte colony stimulating factor (G-CSF) was administrated to further explore if G-CSF can mobilize BM cells and enhance trans-differentiation efficiency of BM cells into renal stem cells.
BM-derived cells can contribute to the Sca-1(+) or c-Kit(+) renal progenitor cells population, although most renal stem cells came from indigenous cells. Furthermore, G-CSF administration nearly doubled the frequency of Sca-1+ BM-derived renal stem cells and increased capillary density of I/R injured kidneys.
These findings indicate that BM derived stem cells can give rise to cells that share properties of renal resident stem cell. Moreover, G-CSF mobilization can enhance this effect.
骨髓（BM）干细胞已被报道可参与肾损伤模型后的组织修复。然而，迄今为止尚无直接证据表明 BM 细胞可转分化为肾干细胞。
为了研究 BM 干细胞是否有助于补充肾干细胞池，我们将表达增强型绿色荧光蛋白（EGFP）的 BM 细胞从转基因小鼠移植到辐射野生型受体中。在血液学重建和缺血再灌注（I/R）后，通过免疫染色和流式细胞术分析评估 Sca-1 和 c-Kit 阳性的肾干细胞。此外，还给予粒细胞集落刺激因子（G-CSF）以进一步探讨 G-CSF 是否可以动员 BM 细胞并提高 BM 细胞向肾干细胞的转分化效率。
BM 来源的细胞可有助于 Sca-1（+）或 c-Kit（+）肾祖细胞群体，尽管大多数肾干细胞来自固有细胞。此外，G-CSF 给药几乎使 Sca-1+BM 衍生的肾干细胞的频率增加了一倍，并增加了 I/R 损伤肾脏的毛细血管密度。
这些发现表明，BM 来源的干细胞可以产生具有肾固有干细胞特性的细胞。此外，G-CSF 动员可以增强这种效应。
