Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), UNLP - CONICET, La Plata, Argentina.
Lett Appl Microbiol. 2010 Jan;50(1):7-12. doi: 10.1111/j.1472-765X.2009.02759.x.
This work aims to investigate the survival of Lactobacillus kefir CIDCA 8348, Lactobacillus plantarum CIDCA 83114 and Saccharomyces lipolytica CIDCA 812, all isolated from kefir, during spray drying and subsequent storage.
Micro-organisms were grown in De Man, Rogosa, Sharpe (MRS) or yeast medium (YM) medium and harvested in the stationary phase of growth. The thermotolerance in skim milk (D and Z values), the survival of spray drying at different outlet air temperatures and subsequent storage in different conditions during 150 days were studied. The resistance to the heat treatments was higher in Lact. plantarum compared to Lact. kefir and S. lipolytica. The three micro-organisms studied varied considerably in their ability to survive to spray drying processes. Lactobacillus plantarum showed the highest survival rate for all the tested outlet air temperatures and also to the further storage in the dried state. The survival rates of Lact. kefir and S. lipolytica through drying and subsequent storage in the dried state decreased when the drying outlet air temperatures increased.
Spray drying is a suitable method to preserve micro-organisms isolated from kefir grains. A high proportion of cells were still viable after 80 days of storage at refrigerated temperatures.
It is the first report about spray-dried probiotic strains isolated from kefir grain and contributes to the knowledge about these micro-organisms for their future application in novel dehydrated products.
本研究旨在考察从克菲尔中分离出的发酵乳杆菌 CIDCA 8348、植物乳杆菌 CIDCA 83114 和解脂耶氏酵母 CIDCA 812 在喷雾干燥和随后储存过程中的存活率。
微生物在德氏乳杆菌培养基（MRS）或酵母培养基（YM）中生长，并在生长的静止期收获。研究了在脱脂乳中的耐热性（D 和 Z 值）、不同出口空气温度下的喷雾干燥存活率以及在 150 天内不同储存条件下的后续储存存活率。与发酵乳杆菌和解脂耶氏酵母相比，植物乳杆菌的耐热性更高。研究的三种微生物在喷雾干燥过程中的存活能力差异很大。植物乳杆菌在所有测试的出口空气温度下表现出最高的存活率，并且在干燥状态下进一步储存时也具有较高的存活率。随着干燥出口空气温度的升高，发酵乳杆菌和解脂耶氏酵母通过干燥和随后在干燥状态下储存的存活率下降。
喷雾干燥是一种保存从克菲尔粒中分离出的微生物的合适方法。在冷藏温度下储存 80 天后，仍有相当比例的细胞保持存活。
这是关于从克菲尔粒中分离出的喷雾干燥益生菌菌株的首次报道，为这些微生物的未来应用于新型脱水产品提供了知识。
