Baracskay Péter, Kiglics Viola, Kékesi Katalin A, Juhász Gábor, Czurkó András
Laboratory of Proteomics, Institute of Biology, Eötvös Loránd University, H-1117 Budapest, Hungary.
BMC Neurosci. 2009 Nov 13;10:133. doi: 10.1186/1471-2202-10-133.
The impairment of the pontine reticular formation (PRF) has recently been revealed to be histopathologically connected with focal-cortical seizure induced generalized convulsive status epilepticus. To elucidate whether the impairment of the PRF is a general phenomenon during status epilepticus, the focal-cortical 4-aminopyridine (4-AP) application was compared with other epilepsy models. The presence of "dark" neurons in the PRF was investigated by the sensitive silver method of Gallyas in rats sacrificed at 3 h after focal 4-AP crystal or systemic 4-AP, pilocarpine, or kainic acid application. The behavioral signs of the developing epileptic seizures were scored in all rats. The EEG activity was recorded in eight rats.
Regardless of the initiating drug or method of administration, "dark" neurons were consistently found in the PRF of animals entered the later phases of status epilepticus. EEG recordings demonstrated the presence of slow oscillations (1.5-2.5 Hz) simultaneously with the appearance of giant "dark" neurons in the PRF.
We argue that the observed slow oscillation corresponds to the late periodic epileptiform discharge phase of status epilepticus, and that the PRF may be involved in the progression of status epilepticus.
最近研究发现脑桥网状结构（PRF）的损伤在组织病理学上与局灶性皮质癫痫诱发的全身惊厥性癫痫持续状态相关。为了阐明PRF的损伤在癫痫持续状态期间是否是一种普遍现象，将局灶性皮质应用4-氨基吡啶（4-AP）与其他癫痫模型进行了比较。通过Gallyas敏感银染法，对在局灶性应用4-AP晶体或全身性应用4-AP、毛果芸香碱或 kainic 酸后3小时处死的大鼠的PRF中“深色”神经元的存在情况进行了研究。对所有大鼠癫痫发作发展的行为体征进行评分。在8只大鼠中记录了脑电图活动。
无论起始药物或给药方法如何，在进入癫痫持续状态后期的动物的PRF中均始终发现“深色”神经元。脑电图记录显示，在PRF中出现巨大“深色”神经元的同时存在慢振荡（1.5 - 2.5 Hz）。
我们认为观察到的慢振荡对应于癫痫持续状态的晚期周期性癫痫样放电阶段，并且PRF可能参与癫痫持续状态的进展。
