Betterle C, Presotto F, Caretto A, Pelizzo M R, Pedini B, Girelli M E, Busnardo B
Institute of Semeiotica Medica, University of Padua, Italy.
Cancer. 1991 Feb 15;67(4):977-83. doi: 10.1002/1097-0142(19910215)67:4<977::aid-cncr2820670420>3.0.co;2-w.
Surgical thyroid sections from 30 papillary carcinomas (PC), six medullary carcinomas (MC), three anaplastic carcinomas (AC), two follicular carcinomas (FC), and 16 adenomas (AD) were examined with an indirect immunofluorescence technique employing different monoclonal antibodies to evaluate the expression of human leukocyte antigen (HLA)-A, B, C (Class I) and DR, DP, DQ (Class II) by thyrocytes, together with the phenotype and distribution of inflammatory cells. Ten PC and four FC were also investigated for the presence of intercellular adhesion molecule-1 (ICAM-1). In situ deposits of immunocomplexes and circulating thyroid autoantibodies were also evaluated. An increased expression of Class I antigens was found in all PC and FC, in 33% of MC and AC, and in 31% of AD. An anomalous expression of Class II antigens was observed in 70% of PC, in 50% of FC, in 33% of AC, in 19% of AD, and in none of the MC. Expression of DP or DQ was revealed only in a portion of the DR-positive glands. A reduction of microsomal autoantigen expression was found. No ICAM-1-positive thyrocytes were detected. A moderate T-lymphocytic infiltrate was noticed only in PC, where it was correlated with DR and DP and/or DQ coexpression. B-cells and natural killer cells were virtually absent. The authors speculate that the weak Class II antigens expression, together with the partial or complete loss in microsomal autoantigen and the absence of ICAM-1 by thyrocytes, may account for the limited engagement of immunocompetent cells observed in thyroid tumors.
采用间接免疫荧光技术，使用不同的单克隆抗体，对30例乳头状癌（PC）、6例髓样癌（MC）、3例未分化癌（AC）、2例滤泡癌（FC）和16例腺瘤（AD）的手术切除甲状腺切片进行检查，以评估甲状腺细胞中人白细胞抗原（HLA）-A、B、C（I类）和DR、DP、DQ（II类）的表达，以及炎症细胞的表型和分布。还对10例PC和4例FC进行了细胞间黏附分子-1（ICAM-1）检测。同时评估了免疫复合物的原位沉积和循环甲状腺自身抗体。结果发现，所有PC和FC、33%的MC和AC以及31%的AD中I类抗原表达增加。70%的PC、50%的FC、33%的AC、19%的AD中观察到II类抗原异常表达，而MC中均未观察到。仅在部分DR阳性腺体中发现DP或DQ表达。发现微粒体自身抗原表达降低。未检测到ICAM-1阳性甲状腺细胞。仅在PC中观察到中度T淋巴细胞浸润，且与DR和DP和/或DQ共表达相关。几乎没有B细胞和自然杀伤细胞。作者推测，II类抗原表达较弱，以及微粒体自身抗原部分或完全缺失，甲状腺细胞缺乏ICAM-1，可能是甲状腺肿瘤中免疫活性细胞参与有限的原因。
