Department of Neurobiology and Behavior, SUNY Stony Brook, Stony Brook, New York 11794 USA.
Mol Cell Neurosci. 1990 Aug;1(1):41-8. doi: 10.1016/1044-7431(90)90040-b.
Neuropeptide Y potently stimulates food intake by acting within the hypothalamus. In this study we examined the possibility that hypothalamic neuropeptide Y expression is increased following food deprivation, as assessed by measuring the hypothalamic content of the mRNA coding for preproneuropeptide Y. Adult male Sprague-Dawley rats were allowed free access to water but were subjected to food withdrawal either overnight or for 72 h or not. Total RNA was isolated from whole hypothalamic dissections and the content of mRNA coding for preproneuropeptide Y was determined by RNase protection analysis. This study revealed a 2.6-fold increase in hypothalamic preproneuropeptide Y mRNA content in 72-h-food- deprived versus control rats with a small increase (40%), which did not reach statistical significance, in overnight-fasted versus control rats. In situ hybridization analysis was used to determine the cellular localization of this increased mRNA content. This study revealed an increase in hybridization in the arcuate nucleus of the hypothalamus of a magnitude similar to that observed using nuclease protection with no change observed over neocortex or reticular nucleus of the thalamus. These data are consistent with the hypothesis that expression of hypothalamic neuropeptide Y is modulated by peripheral metabolic status and support a role for neuropeptide Y in the control of food intake.
神经肽 Y 通过在下丘脑内发挥作用强烈刺激食物摄入。在这项研究中，我们通过测量编码前神经肽 Y 的 mRNA 的下丘脑含量来检查食物剥夺后下丘脑神经肽 Y 表达增加的可能性。成年雄性 Sprague-Dawley 大鼠可以自由饮水，但要么进行过夜或 72 小时的禁食，要么不进行禁食。从整个下丘脑解剖中分离出总 RNA，并通过 RNase 保护分析测定编码前神经肽 Y 的 mRNA 的含量。这项研究显示，与对照组相比，72 小时禁食组大鼠下丘脑前神经肽 Y mRNA 含量增加了 2.6 倍，而 overnight-fasted 组大鼠（增加 40%）则略有增加，但无统计学意义。原位杂交分析用于确定这种增加的 mRNA 含量的细胞定位。这项研究显示，在与使用核酸酶保护观察到的相似程度上，在下丘脑弓状核中观察到杂交增加，而在新皮质或丘脑网状核中没有观察到变化。这些数据与外周代谢状态调节下丘脑神经肽 Y 表达的假说一致，并支持神经肽 Y 在控制食物摄入中的作用。
