Institute of Pathophysiology, Medical Faculty, University of Ljubljana, 61105 Ljubljana, Yugoslavia.
Mol Cell Neurosci. 1991 Jun;2(3):202-7. doi: 10.1016/1044-7431(91)90045-p.
The activities of cholinesterases and choline acetyltransferase in the homogenate and localization of cholinesterases in the ductus deferens of the guinea pig were determined at different times after denervation and decentralization. The activities of choline acetyltransferase and acetylcholinesterase were reduced to about 50% of the control values at all times after denervation; the ultrastructural localization of acetylcholinesterase was not changed. Similar results were obtained for butyryl- and propionylcholinesterase, with the exception at the sixth week after denervation when the activity was not reduced; however, the localization of these enzymes was changed; they appear on the nuclear membrane of the muscle fiber. Our results suggest that the denervation and decentralization of this smooth muscle results not only in changes of activity but also in localization of some cholinesterases. The possibility of a connection between activity of postsynaptically localized enzymes and the permeability of membranes for Ca(2+) is discussed.
在去神经和去神经支配后不同时间，测定豚鼠输精管匀浆中胆碱酯酶和胆碱乙酰转移酶的活性，以及胆碱酯酶在输精管中的定位。去神经后所有时间胆碱乙酰转移酶和乙酰胆碱酯酶的活性均降低至对照值的约 50%；乙酰胆碱酯酶的超微结构定位没有改变。丁酰和丙酰胆碱酯酶也得到了类似的结果，只是在去神经后第六周时，其活性没有降低；然而，这些酶的定位发生了变化；它们出现在肌纤维的核膜上。我们的结果表明，这种平滑肌的去神经和去神经支配不仅导致活性的变化，而且还导致一些胆碱酯酶的定位变化。还讨论了突触后定位酶的活性与 Ca(2+) 通透性之间的可能联系。
