Majcen Z
Histochemistry. 1984;81(2):195-9. doi: 10.1007/BF00490117.
Several authors have reported that longitudinal and circular muscle layers of the guinea-pig ductus deferens possess a rich adrenergic innervation. Cholinergic innervation has been doubted by several authors, especially its presence in the longitudinal muscle layer. Acetylcholinesterase and butyrylcholinesterase were demonstrated, by electron-microscopic examination of both muscle layers of the guinea-pig ductus deferens, to be localized on the axolemma of the nerve endings and in smooth-muscle fibres, on sarcolemma, in the intracellular caveolae and in the intercellular space. Activity of cholinesterases and choline acetyltransferase was measured by the radiometric method and was found in both muscle layers. The activity of butyrylcholinesterase was higher than that of acetylcholinesterases in the homogenates of the whole ductus deferens and in the longitudinal muscle layer. In the circular muscle layer, the activity of acetylcholinesterase was higher than the activity of butyrylcholinesterase. In both muscle layers, we also found choline acetyltransferase, the activity being stronger in the circular layer. The localization of cholinesterases in smooth-muscles in the same places as the calcium and muscarinic acetylcholine receptors is discussed, together with the possibility that the enzyme is in some way involved in the excitation-contraction mechanism of smooth muscle.
几位作者报告称，豚鼠输精管的纵行肌层和环行肌层拥有丰富的肾上腺素能神经支配。胆碱能神经支配受到了几位作者的质疑，尤其是其在纵行肌层中的存在。通过对豚鼠输精管的两层肌肉进行电子显微镜检查，发现乙酰胆碱酯酶和丁酰胆碱酯酶定位于神经末梢的轴膜上、平滑肌纤维上、肌膜上、细胞内小窝中和细胞间隙中。采用放射测量法测定了胆碱酯酶和胆碱乙酰转移酶的活性，结果在两层肌肉中均检测到。在整个输精管匀浆和纵行肌层中，丁酰胆碱酯酶的活性高于乙酰胆碱酯酶。在环行肌层中，乙酰胆碱酯酶的活性高于丁酰胆碱酯酶。在两层肌肉中，我们还发现了胆碱乙酰转移酶，其活性在环行层中更强。文中讨论了胆碱酯酶与钙和毒蕈碱型乙酰胆碱受体在平滑肌中的相同位置定位，以及该酶可能以某种方式参与平滑肌兴奋收缩机制的可能性。
