Department of Neurology, Columbia University, New York, New York 10032.
Mol Cell Neurosci. 1993 Feb;4(1):30-5. doi: 10.1006/mcne.1993.1004.
We have quantified degenerating cells during the postnatal development of the substantia nigra pars compacta (SNpc) of normal rat. The cells are identified at the light microscope level by the characteristic morphologic appearance of large, rounded clumps of chromatin, stained intensely with both Nissl and silver stains. These cells were most numerous on Postnatal Day (PND) 2 and rapidly declined in number by PND 12. However, on PND 14, there was a second peak in the number of pyknotic cells. Their number again rapidly diminished to very few by PND 28. Many of these cells appear neuronal in origin. We conclude that naturally occurring cell death is a prominent feature of normal nigra development, and we hypothesize that the magnitude of this regressive event may be regulated by target (striatal)-derived trophic support.
我们已经量化了正常大鼠黑质致密部（SNpc）出生后发育过程中的退化细胞。这些细胞在光镜下通过大而圆的染色质团块的特征形态外观来识别，用尼氏染色和银染色均可强烈染色。这些细胞在出生后第 2 天（PND2）数量最多，并在 PND12 时迅速减少。然而，在 PND14 时，出现了第二个细胞固缩高峰。它们的数量再次迅速减少到 PND28 时非常少。这些细胞中的许多似乎起源于神经元。我们得出结论，自然发生的细胞死亡是正常黑质发育的一个显著特征，我们假设这种退行性事件的程度可能受到靶（纹状体）来源的营养支持的调节。
