Department of Molecular Pharmacology and Toxicology, University of Southern California School of Pharmacy, 1985 Zonal Avenue, Los Angeles, California 90033.
Mol Cell Neurosci. 1993 Feb;4(1):36-46. doi: 10.1006/mcne.1993.1005.
The rapid effect of 17beta-estradiol upon filopodial growth was investigated using videomicroscopy of hippocampal neurons in culture. Within 1-10 min, 17beta-estradiol induced a significant increase in the number of filopodia decorating neuritic extensions. In addition, the length of existing as well as newly formed filopodia increased significantly during the 10-min 17beta-estradiol exposure. Neurite area and neurite length did not change significantly. The inactive epimer, 17alpha-estradiol, had no effect upon any of the morphological parameters assessed. Similarly, neither progesterone or corticosterone showed a significant influence upon any of the parameters of hippocampal nerve cell growth in culture within the same time frame in which 17beta-estradiol effects were observed. Testosterone induced a significant increase in the number of filopodia without a significant increase in the overall length of the filopodia. Results of these studies demonstrate that 17beta-estradiol can induce rapid changes in nerve cell morphology, suggesting that modification of neuronal circuitry can occur within minutes of exposure, a time base that is consistent with 17beta-estradiol influence upon nerve cell excitability.
使用培养的海马神经元的录像显微镜研究了 17β-雌二醇对丝状伪足生长的快速作用。在 1-10 分钟内，17β-雌二醇诱导突起上的丝状伪足数量显著增加。此外，在 17β-雌二醇暴露的 10 分钟内，现有和新形成的丝状伪足的长度也显著增加。神经突面积和神经突长度没有明显变化。非活性对映体 17α-雌二醇对评估的任何形态参数均无影响。同样，在观察到 17β-雌二醇作用的相同时间范围内，孕激素或皮质酮对培养的海马神经细胞生长的任何参数均无显著影响。睾酮诱导丝状伪足数量显著增加，但丝状伪足的总长度没有显著增加。这些研究的结果表明，17β-雌二醇可以诱导神经细胞形态的快速变化，这表明神经元回路的修饰可以在暴露后的几分钟内发生，这一时间范围与 17β-雌二醇对神经细胞兴奋性的影响一致。
