Department of Anatomy and Cell Biology and Center for Reproductive| Sciences, Columbia University, College of Physicians and Surgeons, New York, New York 10032.
Mol Cell Neurosci. 1993 Dec;4(6):510-25. doi: 10.1006/mcne.1993.1063.
Estrogen and the neurotrophins regulate development, survival, and plasticity of the nervous system. We have shown previously that neurons of the developing basal forebrain and their cortical and hippocampal targets express estrogen receptor mRNA and protein. Furthermore, subsets of neurons within these regions colocalize mRNAs for neurotrophin receptors (p75(NGFR), (trk) A, and (trk)B) and their cognate ligands (NGF, BDNF, and NT-3). Using combined isotopic/nonisotopic in situ hybridization histochemistry, we now demonstrate that mRNAs for the neurotrophins as well as their receptors colocalize to individual estrogen receptor mRNA-containing neurons in these regions of the developing rodent forebrain. The patterns of colocalization were both region and mRNA specific. These results suggest a potential for interactions between estrogen and the neurotrophins, including possible estrogen-stimulated, neurotrophin-mediated autocrine mechanisms that may regulate neuronal differentiation and survival during development.
雌激素和神经营养因子调节神经系统的发育、存活和可塑性。我们之前已经表明，基底前脑的发育中的神经元及其皮质和海马的靶标表达雌激素受体 mRNA 和蛋白。此外，这些区域内的神经元亚群共表达神经营养因子受体（p75(NGFR)、(trk)A 和 (trkB)）及其相应配体（NGF、BDNF 和 NT-3）的 mRNA。现在，我们使用联合同位素/非同位素原位杂交组织化学方法，证明了神经营养因子及其受体的 mRNA 与这些发育中的啮齿动物前脑区域中含有雌激素受体 mRNA 的单个神经元共定位。共定位的模式既具有区域特异性，也具有 mRNA 特异性。这些结果表明雌激素和神经营养因子之间存在相互作用的潜力，包括可能的雌激素刺激、神经营养因子介导的自分泌机制，这些机制可能调节发育过程中的神经元分化和存活。
