Maas Miriam, Van Rhijn Ildiko, Allsopp Maria T E P, Rutten Victor P M G
Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Vet Immunol Immunopathol. 2010 Apr 15;134(3-4):296-8. doi: 10.1016/j.vetimm.2009.10.001. Epub 2009 Oct 12.
Cloning and sequencing of the full length lion and cheetah interferon-gamma (IFN-gamma) transcript will enable the expression of the recombinant cytokine, to be used for production of monoclonal antibodies and to set up lion and cheetah-specific IFN-gamma ELISAs. These are relevant in blood-based diagnosis of bovine tuberculosis, an important threat to lions in the Kruger National Park. Alignment of nucleotide and amino acid sequences of lion and cheetah and that of domestic cats showed homologies of 97-100%.
克隆和测序全长狮子和猎豹干扰素-γ（IFN-γ）转录本，将能够表达重组细胞因子，用于生产单克隆抗体，并建立狮子和猎豹特异性IFN-γ酶联免疫吸附测定（ELISA）。这些对于基于血液的牛结核病诊断具有重要意义，牛结核病是克鲁格国家公园狮子面临的一项重大威胁。狮子、猎豹与家猫的核苷酸和氨基酸序列比对显示，它们之间的同源性为97%-100%。
