Dorner Brigitte G, Dorner Martin B, Zhou Xuefei, Opitz Corinna, Mora Ahmed, Güttler Steffen, Hutloff Andreas, Mages Hans W, Ranke Katja, Schaefer Michael, Jack Robert S, Henn Volker, Kroczek Richard A
Molecular Immunology, Robert Koch-Institute, 13353 Berlin, Germany.
Immunity. 2009 Nov 20;31(5):823-33. doi: 10.1016/j.immuni.2009.08.027. Epub 2009 Nov 12.
The expression of the chemokine receptor XCR1 and the function of its ligand XCL1 (otherwise referred to as ATAC, lymphotactin, or SCM-1) remained elusive to date. In the present report we demonstrated that XCR1 is exclusively expressed on murine CD8(+) dendritic cells (DCs) and showed that XCL1 is a potent and highly specific chemoattractant for this DC subset. CD8(+) T cells abundantly secreted XCL1 8-36 hr after antigen recognition on CD8(+) DCs in vivo, in a period in which stable T cell-DC interactions are known to occur. Functionally, XCL1 increased the pool of antigen-specific CD8(+) T cells and their capacity to secrete IFN-gamma. Absence of XCL1 impaired the development of cytotoxicity to antigens cross-presented by CD8(+) DCs. The XCL1-XCR1 axis thus emerges as an integral component in the development of efficient cytotoxic immunity in vivo.
趋化因子受体XCR1的表达及其配体XCL1（也称为ATAC、淋巴细胞趋化因子或SCM-1）的功能至今仍不清楚。在本报告中，我们证明XCR1仅在小鼠CD8(+)树突状细胞（DCs）上表达，并表明XCL1是该DC亚群的一种强效且高度特异性的趋化因子。在体内，CD8(+)T细胞在识别CD8(+)DCs上的抗原后8 - 36小时大量分泌XCL1，而这正是已知会发生稳定的T细胞 - DC相互作用的时期。在功能上，XCL1增加了抗原特异性CD8(+)T细胞的数量及其分泌γ干扰素的能力。缺乏XCL1会损害对CD8(+)DCs交叉呈递抗原的细胞毒性的发展。因此，XCL1 - XCR1轴成为体内有效细胞毒性免疫发展的一个不可或缺的组成部分。
