Blond McIndoe Research Laboratories, Tissue Injury and Repair Group, University of Manchester, UK.
Brain Res. 2010 Jan 15;1310:1-7. doi: 10.1016/j.brainres.2009.11.011. Epub 2009 Nov 11.
Peripheral nerve injury leads to deficient recovery of sensation and a causative factor may be that only 50-60% of primary sensory neurons succeed in regenerating axons after primary nerve repair. In this study, an in vivo rat sciatic nerve injury and regeneration model was combined with laser microdissection and quantitative real-time polymerase chain reaction with the aim of examining the gene expression of regenerative molecules in cutaneous and muscular sensory neurons. Recent studies have identified peripherin and ATF-3 molecules as crucial for neurite outgrowth propagation; our novel findings demonstrate a subpopulation of non-regenerating sensory neurons characterized by a failure to upregulate transcription of these molecules and that a greater peripherin mRNA expression in injured cutaneous neurons may potentiate this subpopulation to regenerate more axons than muscle afferent neurons following injury. The gene expression of the structural neurofilament NF-H is found to be significantly downregulated following injury in both sensory subpopulations.
周围神经损伤导致感觉恢复不足，一个原因可能是只有 50-60%的初级感觉神经元在初级神经修复后成功再生轴突。在这项研究中，我们将体内大鼠坐骨神经损伤和再生模型与激光显微切割和实时定量聚合酶链反应相结合，旨在检测皮肤和肌肉感觉神经元中再生分子的基因表达。最近的研究已经确定 peripherin 和 ATF-3 分子对轴突生长的传播至关重要；我们的新发现表明，存在一个不能上调这些分子转录的非再生感觉神经元亚群，而且受伤的皮肤神经元中更多的 peripherin mRNA 表达可能使这个亚群比肌肉传入神经元在受伤后再生更多的轴突。在这两个感觉亚群中，结构神经丝 NF-H 的基因表达在损伤后均显著下调。
