Department of Internal Medicine I, University Hospital of Regensburg, Regensburg, Germany.
Metabolism. 2010 May;59(5):664-70. doi: 10.1016/j.metabol.2009.09.011. Epub 2009 Nov 14.
No detailed data are available on hepatic clearance, postprandial release, and distribution profile of metabolically active adipokines in splanchnic blood compartments such as portal and hepatic veins. This would be a prerequisite for understanding the role of visceral adipose tissue-derived adipokines in metabolism. Adiponectin, resistin, leptin, and visfatin concentrations were measured by enzyme-linked immunosorbent assay in peripheral veins, arterial blood, hepatic veins, and portal veins in 50 patients with liver cirrhosis undergoing transjugular intrahepatic portosystemic shunt implantation, in 6 patients with normal liver function, and in fasted and fed rats. Adiponectin, leptin, resistin, and visfatin did not differ among blood compartments in normal-weight probands in the fasted state. Adiponectin and leptin levels were similar in patients with and without liver cirrhosis. Systemic visfatin levels were decreased and resistin levels were increased in liver cirrhosis. Visfatin secretion was higher from visceral than from peripheral subcutaneous adipose tissue in liver cirrhosis. There was no hepatic clearance of visfatin. Leptin secretion was higher from peripheral than from visceral adipose tissue. Leptin did not undergo hepatic clearance. Resistin and adiponectin did not differ between blood compartments in liver cirrhosis. Resistin concentrations increased upon feeding in rats, and there was an increase in the postprandial clearance of adiponectin by the liver. A postprandial increase of leptin concentrations was restricted to peripheral adipose tissue in rats. The results give insight into the dynamics of splanchnic adipokine concentrations and help critically interpret data derived from messenger RNA expression studies.
尚无详细数据可说明门脉和肝静脉等内脏血液中代谢活跃的脂肪因子的肝清除率、餐后释放和分布特征。这是了解内脏脂肪组织来源的脂肪因子在代谢中的作用的前提条件。采用酶联免疫吸附试验检测 50 例肝硬化经颈静脉肝内门体分流术（TIPS）植入患者、6 例肝功能正常患者及禁食和进食大鼠的外周静脉、动脉血、肝静脉和门静脉中的脂联素、抵抗素、瘦素和内脂素浓度。在禁食状态下，正常体重的志愿者各血液隔间中的脂联素、瘦素、抵抗素和内脂素均无差异。肝硬化患者和无肝硬化患者的脂联素和瘦素水平相似。肝硬化患者全身内脂素水平降低，抵抗素水平升高。肝硬化时，内脏脂肪组织比外周皮下脂肪组织的内脂素分泌更高。内脂素无肝清除。瘦素从外周脂肪组织的分泌高于从内脏脂肪组织。瘦素无肝清除。肝硬化患者各血液隔间中的抵抗素和脂联素无差异。在大鼠中，抵抗素浓度在进食后增加，且肝脏对脂联素的餐后清除率增加。大鼠外周脂肪组织中瘦素浓度的餐后增加受到限制。这些结果深入了解了内脏脂肪因子浓度的动力学，有助于批判性地解释来自信使 RNA 表达研究的数据。
