National Center of Radiobiology and Radiation Protection, 1756 Sofia, Bulgaria.
Mutat Res. 2010 Jan;695(1-2):40-5. doi: 10.1016/j.mrgentox.2009.11.002. Epub 2009 Nov 13.
This study analyzed the effects of biliprotein C-phycocyanin (C-PC) on the enzymatic antioxidant defence system in lymphocytes of nuclear power-plant workers and non-exposed controls. Changes in the protein levels of manganese super oxide dismutase (MnSOD), catalase and glutathione-S-transferase (GST) were used as markers for early biological effects of a single in vitro exposure of cells to: (i) 2Gy gamma rays; (ii) 5muM C-PC; and (iii) a combination of C-PC plus irradiation with 2Gy. The results showed that C-PC selectively stimulated the lymphocyte antioxidant defence system of occupationally exposed subjects. The activation of the antioxidant protective mechanisms as part of the early radiation response was probably related to the chronic low-dose occupational exposure. The modulating capacity of C-PC at the molecular level may be of interest for the protection of occupationally exposed persons.
本研究分析了藻蓝蛋白（C-PC）对核电站工作人员和非暴露对照组淋巴细胞酶抗氧化防御系统的影响。锰超氧化物歧化酶（MnSOD）、过氧化氢酶和谷胱甘肽-S-转移酶（GST）的蛋白质水平变化被用作细胞单次体外暴露于以下物质的早期生物学效应的标志物：（i）2Gy γ射线；（ii）5μM C-PC；和（iii）C-PC 加 2Gy 辐射的组合。结果表明，C-PC 选择性地刺激了职业暴露受试者的淋巴细胞抗氧化防御系统。作为早期辐射反应的一部分，抗氧化保护机制的激活可能与慢性低剂量职业暴露有关。C-PC 在分子水平上的调节能力可能对职业暴露人群的保护具有重要意义。
