Sivasankari Sivaprakasam, Vinoth Mani, Ravindran David, Baskar Kathirvelu, Alqarawi Abdulaziz A, Abd Allah Elsayed Fathi
Department of Biology, Gandhigram Rural Institute-DU, Gandhigram, Dindigul, Tamil Nadu, 624302, India.
Research Department of Botany, Jamal Mohamed College, Tiruchirappalli, Tamil Nadu, 620020, India.
Bioprocess Biosyst Eng. 2021 Jan;44(1):141-150. doi: 10.1007/s00449-020-02430-5. Epub 2020 Sep 4.
In this study, red LED and urea used as light and nitrogen sources, respectively, for the cultivation of Spirulina to enhance the fluorescence property and purity of phycocyanin. Besides, there is a high concentration of phycocyanin leached out from red light (RL) grown cells than white light (WL) without cell disruption. This type of cultivation reduces the complexity of extraction methods and cost of the downstream process. The fluorescence intensity of C-PC enhanced while using red LEDs and purity ratio improved by single-step cation exchange chromatography. Phycocyanin from red-light-exposed culture exhibited pronounced antibacterial activity against bacteria. The hydrogen peroxide scavenging activity of C-PC (93.7%) is higher than the WL cultures (88.8%). Phycocyanin from RL culture exhibited a strong antiproliferative activity (64.1%) against HeLa cancer cell line. The present study aims to analyze the influence of red light and urea on enhancing the phycocyanin production.
在本研究中，分别使用红色发光二极管（LED）和尿素作为光源和氮源来培养螺旋藻，以增强藻蓝蛋白的荧光特性和纯度。此外，在不进行细胞破碎的情况下，与白光（WL）培养的细胞相比，红光（RL）培养的细胞中渗出的藻蓝蛋白浓度更高。这种培养方式降低了提取方法的复杂性和下游工艺的成本。使用红色LED时，C-藻蓝蛋白（C-PC）的荧光强度增强，并且通过单步阳离子交换色谱法提高了纯度比。来自红光照射培养物的藻蓝蛋白对细菌表现出显著的抗菌活性。C-PC的过氧化氢清除活性（93.7%）高于白光培养物（88.8%）。来自红光培养物的藻蓝蛋白对HeLa癌细胞系表现出较强的抗增殖活性（64.1%）。本研究旨在分析红光和尿素对提高藻蓝蛋白产量的影响。
