School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
Biosens Bioelectron. 2010 Jan 15;25(5):1160-5. doi: 10.1016/j.bios.2009.10.004. Epub 2009 Nov 14.
An amperometric biosensor for the detection of the reduced nicotinamide cofactors NADH and NADPH was designed, based on the electrochemical oxidation of NAD(P)H with an iron oxide/carbon black composite (Fe(2)O(3)/CB) electrode. The electrode exhibited excellent performances in that it led to a substantial decrease in the overpotential of electrochemical NADH oxidation. Iron oxide plays a significant role as a catalyst for NADH oxidation and the reaction occurs at +0.00 V (vs. Ag/AgCl). The method of the sensor construction is very simple and the sensor performed well, giving high sensitivity, high stability, and a broad detection range. The sensitivity of this system is 2.54 microA mM(-1) and the limit of detection (S/N=3) is 10 microM. A linear range was observed between 10 microM and 1000 microM of NADH (R(2)=0.993), which is preferable to that of the previous studies. The Fe(2)O(3)/CB electrode also oxidizes NADPH under the same condition and can be applied as an NADPH sensor. Moreover, when the sensor system was integrated into a dehydrogenase-based sensor system, it also showed a good sensing performance.
基于铁氧化物/碳黑复合材料（Fe(2)O(3)/CB）电极对烟酰胺腺嘌呤二核苷酸（NADH）和烟酰胺腺嘌呤二核苷酸磷酸（NADPH）的电化学氧化作用，设计了一种用于检测还原型烟酰胺辅酶 NADH 和 NADPH 的电流型生物传感器。该电极具有出色的性能，能显著降低电化学 NADH 氧化的过电势。铁氧化物在 NADH 氧化反应中起到了重要的催化作用，反应在 +0.00 V（相对于 Ag/AgCl）发生。传感器的构建方法非常简单，且传感器性能良好，具有高灵敏度、高稳定性和宽检测范围。该系统的灵敏度为 2.54 μA mM(-1)，检测限（S/N=3）为 10 μM。在 10 μM 至 1000 μM 的 NADH 范围内观察到线性范围（R(2)=0.993），优于先前的研究。在相同条件下，Fe(2)O(3)/CB 电极也能氧化 NADPH，可作为 NADPH 传感器。此外，当将传感器系统集成到基于脱氢酶的传感器系统中时，它也表现出良好的传感性能。
