原发性干燥综合征患者外周血中浆细胞样树突状细胞和 2 型髓系树突状细胞的水平降低。
Levels of plasmacytoid dendritic cells and type-2 myeloid dendritic cells are reduced in peripheral blood of patients with primary Sjogren's syndrome.
机构信息
Broegelmann Research Laboratory, The Gade Institute, University of Bergen, Laboratory building, 5th floor, N-5021 Bergen, Norway.
出版信息
Ann Rheum Dis. 2010 Jun;69(6):1235-8. doi: 10.1136/ard.2009.118158. Epub 2009 Nov 12.
OBJECTIVE
Sjögren's syndrome (SS) is a lymphoproliferative autoimmune disease, characterised by dryness of the mouth and eyes. Dendritic cells (DC) are potent antigen-presenting cells crucial for initiating and maintaining primary immune responses. This study quantified interferon-producing plasmacytoid DC (pDC) and two myeloid DC subsets (mDC1 and mDC2) in peripheral blood (PB) from primary SS (pSS) patients and healthy controls.
METHODS
Blood samples from 31 pSS patients and 28 gender and age-matched healthy controls were analysed by flow cytometry using the Miltenyi Blood DC enumeration kit. The presence of pDC in salivary glands (SG) from pSS patients was analysed by immunohistochemistry.
RESULTS
Patients with pSS had significantly less pDC and mDC2 in PB compared with healthy controls. Moreover, pDC are present in SG from patients with pSS.
CONCLUSION
Patients with pSS have alterations among DC populations in PB, and pDC are present in the SG, suggesting a potential role of these cells in SS.
目的
干燥综合征（SS）是一种淋巴增生性自身免疫性疾病，其特征为口眼干燥。树突状细胞（DC）是启动和维持初级免疫反应的有效抗原呈递细胞。本研究旨在定量检测原发性 SS（pSS）患者和健康对照者外周血中的干扰素产生型浆细胞样 DC（pDC）和两种髓样 DC 亚群（mDC1 和 mDC2）。
方法
采用 Miltenyi Blood DC 计数试剂盒，通过流式细胞术分析 31 例 pSS 患者和 28 例性别和年龄匹配的健康对照者的血液样本。采用免疫组织化学法分析 pSS 患者唾液腺（SG）中的 pDC。
结果
与健康对照者相比，pSS 患者的外周血 pDC 和 mDC2 明显减少。此外，pSS 患者的 SG 中存在 pDC。
结论
pSS 患者外周血中的 DC 群体发生改变，pDC 存在于 SG 中，提示这些细胞在 SS 中可能具有潜在作用。
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