Dipartimento di Medicina del Lavoro, Università degli Studi di Milano e Fondazione IRCCS, Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Milan, Italy.
Occup Environ Med. 2010 Jul;67(7):456-63. doi: 10.1136/oem.2009.050344. Epub 2009 Nov 12.
To assess dermal exposure to 16 polycyclic aromatic hydrocarbons (PAHs) in asphalt workers by applying polypropylene pads to six body sites (neck, shoulder, upper arm, wrist, groin, ankle), to identify the compounds and exposure sites most representative, and to integrate dermal exposure results with environmental and biological data.
Twenty-four asphalt workers were recruited. Dermal exposure was assessed during a single work shift. Sixteen PAHs (from naphthalene to indeno[1,2,3-cd]pyrene) were quantified via gas chromatography-mass spectrometry. Airborne exposure, urinary PAHs and monohydroxy metabolites were also investigated.
Phenanthrene (PHE), present in all samples, was the most abundant compound (median 0.805-1.825 ng/cm(2)). Benzo[a]pyrene (BaP) was present in 75% of the samples (0.046-0.101 ng/cm(2)). Wrist had the highest contamination, with median PHE, pyrene (PYR), and BaP concentrations of 1.825, 0.527, and 0.063 ng/cm(2). PHE and PYR on wrist correlated with almost all 3- to 4-ring PAHs (0.405< or =r< or =0.856), but not with BaP; BaP correlated with almost all 4- to 6- ring PAHs (0.584< or =r< or =0.633). Significant correlations were observed between PHE level, airborne exposure, and the corresponding urinary PHE and monohydroxy metabolites. For PYR, significant correlations existed only between urinary PYR and monohydroxy metabolites. Multiple linear regression analysis revealed that 42% of the end-of-shift monohydroxy metabolites were the result of airborne exposure, dermal exposure, and baseline levels of biomarkers.
Dermal exposure to PAHs was in the low ng/cm(2) range. PHE or PYR and BaP were the most representative compounds and the wrist was the best location to perform dermal exposure assessments. Both dermal and airborne exposure contributed to the total body burden of PAHs, though the relative contribution was analyte-dependent.
通过在六个身体部位（颈部、肩部、上臂、手腕、腹股沟、脚踝）应用聚丙烯垫来评估沥青工人接触 16 种多环芳烃（PAHs）的皮肤暴露情况，确定最具代表性的化合物和暴露部位，并将皮肤接触结果与环境和生物数据整合。
招募了 24 名沥青工人。在单个工作班次期间评估皮肤接触情况。通过气相色谱-质谱法对 16 种 PAHs（萘到茚并[1,2,3-cd]芘）进行定量分析。还调查了空气中的暴露、尿液中的 PAHs 和单羟基代谢物。
所有样本中均存在菲（PHE），是最丰富的化合物（中位数 0.805-1.825ng/cm(2)）。苯并[a]芘（BaP）存在于 75%的样本中（0.046-0.101ng/cm(2)）。手腕污染最严重，PHE、苊（PYR）和 BaP 的浓度中位数分别为 1.825、0.527 和 0.063ng/cm(2)。手腕上的 PHE 和 PYR 与几乎所有 3-4 环 PAHs （0.405<=r<=0.856）相关，但与 BaP 无关；BaP 与几乎所有 4-6 环 PAHs（0.584<=r<=0.633）相关。在 PHE 水平、空气暴露和相应的尿液 PHE 和单羟基代谢物之间观察到显著相关性。对于 PYR，仅在尿液 PYR 和单羟基代谢物之间存在显著相关性。多元线性回归分析表明，42%的轮班结束时的单羟基代谢物是空气暴露、皮肤暴露和生物标志物基线水平的结果。
PAHs 的皮肤接触处于低 ng/cm(2)范围。PHE 或 PYR 和 BaP 是最具代表性的化合物，手腕是进行皮肤接触评估的最佳部位。皮肤暴露和空气暴露均有助于 PAHs 的全身负担，但其相对贡献取决于分析物。
