The toxicities of phenanthrene (PH) and pyrene (PY) are less than benzo (a) pyrene (BaP) , but both compounds are found in higher concentrations in the air, feed, and food. Most PAHs are metabolized to hydroxylated compounds by the hepatic cytochrome P450 monooxigenases system. Metabolites are excreted into urine and feces. We determined concentrations of PH, PY and BaP in muscle and hydroxylated metabolites, 3-OH-PH, 1-OH-PY, and 3-OH-BaP, respectively, in urine from dairy cattle (n = 24) . We also evaluated the relationship between parent compounds in muscle and their metabolites in urine. Concentrations of PH and PY in muscle ranged from 0.74.8 ng/g (1.8 ± 1.7) and 0.44.1 ng/g (1.2 ± 1.2) , respectively. Concentrations of 3-OH-PH and 1-OH-PY in urine ranged from 0.15.9 ng/ml (2.9 ± 3.7) and 0.53.6 ng/ml (1.9 ± 2.3) , respectively. Correlation coefficient for PY concentration in muscle versus 1-OH-PY in urine was 0.657 and for PH concentration in muscle versus 3-OH-PH in urine was 0.579.Coefficient determination for PY and PH concentrations in muscle was 0.886 and for 1-OH-PY and 3-OHPH in urine was 0.834. This study suggests that 1-OH-PY and 3-OH-PH could be used as biomarkers for PAHs exposure in dairy cattle.