Okutani Akiko, Sekizuka Tsuyoshi, Boldbaatar Bazartseren, Yamada Akio, Kuroda Makoto, Inoue Satoshi
Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo, Japan.
J Vet Med Sci. 2010 Jan;72(1):93-7. doi: 10.1292/jvms.09-0213. Epub 2009 Nov 13.
Twelve strains of Bacillus anthracis isolated in Japan were subjected to multiple locus variable-number tandem repeats analysis using 25 marker loci (MLVA25). The results showed that Japanese strains could be divided into two distinct genetic clusters, A3a and A3b. By using newly devised comprehensive single nucleotide polymorphisms (SNPs) analysis, Japanese strains were also divided into two groups. The results obtained by the MLVA25 and plasmids SNP analysis well coincided, indicating that both methods were highly sensitive to discriminate B. anthracis strains. These results suggested that MLVA25 had sufficient discrimination power to identify B. anthracis at the strain level, and that MLVA25 as well as comprehensive SNPs analysis could facilitate further studies of B. anthracis strains including Japanese and other Asian strains.
对在日本分离出的12株炭疽芽孢杆菌进行了多位点可变数目串联重复序列分析（MLVA），使用25个标记位点（MLVA25）。结果表明，日本菌株可分为两个不同的遗传簇，A3a和A3b。通过新设计的综合单核苷酸多态性（SNP）分析，日本菌株也被分为两组。MLVA25和质粒SNP分析得到的结果高度吻合，表明这两种方法在区分炭疽芽孢杆菌菌株方面都具有高度敏感性。这些结果表明，MLVA25在菌株水平上具有足够的鉴别力来鉴定炭疽芽孢杆菌，并且MLVA25以及综合SNP分析有助于对包括日本和其他亚洲菌株在内的炭疽芽孢杆菌菌株进行进一步研究。
