Toriyama K, Thorsness M K, Nasrallah J B, Nasrallah M E
Division of Biological Sciences, Cornell University, Ithaca, New York 14853.
Dev Biol. 1991 Feb;143(2):427-31. doi: 10.1016/0012-1606(91)90094-j.
The S locus glycoprotein (SLG) gene of Brassica encodes stigmatic glycoproteins that are implicated in the pollen-stigma interaction of self-incompatibility. We have transformed the related plant Arabidopsis thaliana with a chimaeric gene consisting of the promoter region of an SLG gene fused to the reporter gene beta-glucuronidase (GUS). In transgenic plants the gene was expressed in two cell types of the flower. In stigmas, the timing and distribution of GUS activity was similar to that previously described for SLG expression in Brassica. In anthers, expression was detected at an earlier stage of flower development with GUS activity restricted to the tapetal cell layer. The novel finding of SLG-promoter activity in the anther supports the hypothesis that sporophytic control of self-incompatibility is a result of SLG-gene expression in the tapetum.
芸苔属植物的S位点糖蛋白（SLG）基因编码柱头糖蛋白，这些糖蛋白参与了自交不亲和的花粉-柱头相互作用。我们用一个嵌合基因转化了相关植物拟南芥，该嵌合基因由一个SLG基因的启动子区域与报告基因β-葡萄糖醛酸酶（GUS）融合而成。在转基因植物中，该基因在花的两种细胞类型中表达。在柱头中，GUS活性的时间和分布与之前描述的芸苔属植物中SLG的表达情况相似。在花药中，在花发育的早期阶段检测到表达，GUS活性局限于绒毡层细胞层。花药中SLG启动子活性这一新发现支持了这样一种假说，即自交不亲和的孢子体控制是绒毡层中SLG基因表达的结果。
