Instituto de Biología Molecular y Celular de Plantas CSIC-UPV, Campus de la Universidad Politécnica de Valencia, Valencia, Spain.
CREA Research Centre for Genomics and Bioinformatics, Fiorenzuola d'Arda, Italy.
Methods Mol Biol. 2023;2686:241-259. doi: 10.1007/978-1-0716-3299-4_10.
Carpels are the female reproductive organs of the flower, organized in a gynoecium, which is likely the most complex organ of the plant. The gynoecium provides protection for the ovules, helps to discriminate between male gametophytes, and facilitates successful pollination. After fertilization, it develops into a fruit, a specialized organ for seed protection and dispersal. To carry out all these functions, coordinated patterning and tissue specification within the developing gynoecium has to be achieved. In this chapter, we provide different methods to characterize defects in carpel morphogenesis and patterning associated with developmental mutations, as well as a list of reporter lines that can be used to facilitate genetic analyses.
心皮是花的雌性生殖器官，组织成雌蕊，它可能是植物中最复杂的器官。雌蕊为胚珠提供保护，有助于区分雄性配子体，并促进成功授粉。受精后，它发育成果实，是一种专门用于保护和传播种子的器官。为了执行所有这些功能，在发育中的雌蕊中必须实现协调的模式形成和组织特化。在本章中，我们提供了不同的方法来描述与发育突变相关的心皮形态发生和模式缺陷，并列出了可用于促进遗传分析的报告基因系。
