Albrecht von Haller Institute for Plant Sciences, Plant Biochemistry, Göttingen University, 37077 Göttingen, Germany.
Planta. 2010 Feb;231(3):507-21. doi: 10.1007/s00425-009-1049-5. Epub 2009 Nov 14.
Datisca glomerata forms nitrogen-fixing root nodules in symbiosis with soil actinomycetes from the genus Frankia. Analysis of sugars in roots, nodules and leaves of D. glomerata revealed the presence of two novel compounds that were identified as alpha-L-rhamnopyranoside-(1 --> 6)-D-glucose (rutinose) and alpha-L-rhamnopyranoside-(1 --> 6)-1-O-beta-D-methylglucose (methylrutinose). Rutinose has been found previously as a/the glycoside part of several flavonoid glycosides, e.g. rutin, also of datiscin, the main flavonoid of Datisca cannabina, but had not been reported as free sugar. Time course analyses suggest that both rutinose and methylrutinose might play a role in transient carbon storage in sink organs and, to a lesser extent, in source leaves. Their concentrations show that they can accumulate in the vacuole. Rutinose, but not methylrutinose, was accepted as a substrate by the tonoplast disaccharide transporter SUT4 from Arabidopsis. In vivo (14)C-labeling and the study of uptake of exogenous sucrose and rutinose from the leaf apoplast showed that neither rutinose nor methylrutinose appreciably participate in phloem translocation of carbon from source to sink organs, despite rutinose being found in the apoplast at significant levels. A model for sugar metabolism in D. glomerata is presented.
滨藜叶猪毛菜与土壤放线菌弗兰克氏菌共生形成固氮根瘤。对滨藜叶猪毛菜的根、瘤和叶中的糖进行分析，发现存在两种新型化合物，它们被鉴定为α-L-鼠李吡喃糖苷-(1→6)-D-葡萄糖（芦丁糖）和α-L-鼠李吡喃糖苷-(1→6)-1-O-β-D-甲基葡萄糖（甲基芦丁糖）。芦丁糖先前曾作为几种类黄酮糖苷的糖苷部分被发现，例如芦丁，也是滨藜叶猪毛菜的主要类黄酮，但其作为游离糖尚未被报道。时程分析表明，芦丁糖和甲基芦丁糖可能在汇器官的暂态碳储存中发挥作用，在源叶中作用较小。它们的浓度表明它们可以在液泡中积累。芦丁糖而非甲基芦丁糖被拟南芥液泡双糖转运蛋白 SUT4 接受为底物。体内（14）C 标记和对外源蔗糖和芦丁糖的摄取研究表明，尽管芦丁糖在质外体中以显著水平存在，但芦丁糖和甲基芦丁糖都不会显著参与从源到汇器官的韧皮部碳转运。提出了滨藜叶猪毛菜糖代谢模型。
