Cermák Pavel, Ptáková Miluse, Kubele Jan, Cermáková Renata, Jedlicková Anna
Thomayer University Hospital with Polyclinic, Prague, Czech Republic.
Klin Mikrobiol Infekc Lek. 2009 Oct;15(5):166-70.
Significant bacteriuria is very important marker of urinary tract infection. The standard method for diagnosis of UTI is quantitative urine culture and identification of bacteria. For rapid enumeration of bacteria in urine semiautomatic systems were developed. Aim of the study was to compare the results of the conventional urine culture method with nephelometric enumeration of bacterial cells in urine.
Urine samples were evaluated simultaneously by Uro-Quick and quantitative urine culture.
In summary 1 653 urine samples were elaborated, 402 (83.05 %) from 484 positive samples had positive results in Uro-Quick. Sensitivity of Uro-Quick was 0.83, specificity 0.95, positive predictive value 0.85, negative predictive value 0.93 and accuracy 90.68. Culture time extension improved statistical performance characteristic of Uro-Quick. Problem of the method is the detection of the low amounts of microorganisms. Uro-Quick did not detected microbial growth in 16.77 % of samples containing >/= ten to the fourth CFU/ml of microorganisms. The advantage of Uro-Quick is the speed, 73.93 % of the samples with high microbial concentrations (>/= ten to the fifth CFU/ml) were detected in three hours.
Uro-Quick offers rapid detection of urine samples with high microbial concentrations, but did not replace the quantitative culture of. Nephelometric detection is necessary to complete with agar plate cultivation.
显著菌尿是尿路感染的重要标志。尿路感染的标准诊断方法是定量尿培养和细菌鉴定。为了快速计数尿液中的细菌，已开发出半自动系统。本研究的目的是比较传统尿培养方法与尿液中细菌细胞比浊法计数的结果。
同时采用Uro-Quick和定量尿培养对尿样进行评估。
总共分析了1653份尿样，484份阳性样本中的402份（83.05%）在Uro-Quick检测中呈阳性结果。Uro-Quick的敏感性为0.83，特异性为0.95，阳性预测值为0.85，阴性预测值为0.93，准确性为90.68。延长培养时间可改善Uro-Quick的统计性能特征。该方法的问题在于检测少量微生物。Uro-Quick在16.77%含有≥10⁴CFU/ml微生物的样本中未检测到微生物生长。Uro-Quick的优点是速度快，73.93%微生物浓度高（≥10⁵CFU/ml）的样本在三小时内被检测出来。
Uro-Quick可快速检测微生物浓度高的尿样，但不能替代定量培养。比浊法检测需要与琼脂平板培养相结合。
