Xu Ming-Shu, Fang Cheng, Xu Jia, Zhang Gen-Feng, Ge Lin-Bao
Shanghai Research Institute of Acu-moxibustion and Meridians, Shanghai 200030, China.
Zhen Ci Yan Jiu. 2009 Aug;34(4):230-5.
To explore the involvement of dopamine (DA) and its metabolites in the extracellular fluid of the striatum on the resisting action of electroacupuncture (EA) for acute cerebral ischemia-reperfusion (CI/R) injury.
A total of 50 SD male rats were randomly divided into normal control, sham-operation (sham), sham + EA, CI/R model, CI/R + EA groups. CI/R model was induced by right middle cerebral artery occlusion (MCAO) for 90 min and reperfusion for 225 min. EA (2 Hz, 3 mA, continuous waves) was applied to "Fengchi" (GB 20) for 20 min, with a 10 min rest, and given once again fo 20 min. The concentrations of DA and its metabolites [3,4- dihydroxy-phenyl acetic acid (DOPAC) and homovanillic acid (HVA)] in the extracellular fluid of the striatum were determined by microdialysis and high performance liquid chromatography (HPLC).
The extracellular levels of DA, DOPAC and HVA in normal group, sham group and sham+ EA group had no significant changes during the whole study. The extracellular level of DA peaked 15-45 min after CI and 0-30 min after CI/R (P < 0.05). A rising trend was also observed 120 min following CI/R. DOPAC level peaked 15 min and 75 min after CI and 75 min after CI/R. After EA, DA level was significantly lower in EA group than that in model group 90 min after CI/R (P < 0.05), and no peak was observed again. After EA, DOPAC level maintained its basic level, and no peak was found after CI/R. The changing trend of HVA was similar to that of DOPAC, and the extracellular HVA level in EA + model group was significantly lower than that in model group 75 min after CI/R.
EA can decrease the accumulation of DA and its metabolites in the extracellular fluid of the striatum, which may contribute to its effect in preventing the brain from CI/R injury.
探讨纹状体细胞外液中多巴胺（DA）及其代谢产物在电针（EA）抗急性脑缺血再灌注（CI/R）损伤中的作用。
将50只雄性SD大鼠随机分为正常对照组、假手术组（sham）、假手术+电针组、CI/R模型组、CI/R+电针组。采用右侧大脑中动脉闭塞（MCAO）90分钟并再灌注225分钟的方法制备CI/R模型。将电针（2Hz，3mA，连续波）施加于“风池”（GB 20）20分钟，休息10分钟后，再次给予20分钟。采用微透析和高效液相色谱（HPLC）法测定纹状体细胞外液中DA及其代谢产物[3,4-二羟基苯乙酸（DOPAC）和高香草酸（HVA）]的浓度。
正常组、假手术组和假手术+电针组在整个研究过程中DA、DOPAC和HVA的细胞外水平无明显变化。CI后15-45分钟和CI/R后0-30分钟DA的细胞外水平达到峰值（P<0.05）。CI/R后120分钟也观察到上升趋势。DOPAC水平在CI后15分钟和75分钟以及CI/R后75分钟达到峰值。电针后，CI/R后90分钟电针组的DA水平显著低于模型组（P<0.05），且未再次出现峰值。电针后，DOPAC水平维持在基础水平，CI/R后未出现峰值。HVA的变化趋势与DOPAC相似，CI/R后75分钟电针+模型组的细胞外HVA水平显著低于模型组。
电针可降低纹状体细胞外液中DA及其代谢产物的蓄积，这可能是其预防脑CI/R损伤作用的机制之一。
