Ramakrishna B S, Roberts-Thomson I C, Pannall P R, Roediger W E
Department of Surgery, Queen Elizabeth Hospital, South Australia.
Gut. 1991 Jan;32(1):46-9. doi: 10.1136/gut.32.1.46.
Substantial amounts of phenols are produced in the human colon by bacterial fermentation of protein. In the colonic mucosa of animals, phenols are inactivated predominantly by conjugation with sulphate. The purpose of this study was to confirm sulphation of phenols by isolated colonocytes from man and to evaluate mucosal sulphation in inflammatory bowel disease using the phenol, paracetamol, in rectal dialysis bags. The incubation of paracetamol with colonocytes isolated from resected colon specimens (n = 7) yielded a mean (SE) value of 7.0 (0.9) mumols/g dry weight of paracetamol sulphate after 60 minutes but virtually undetectable values of paracetamol glucuronide. Paracetamol sulphate was detected in rectal dialysates from all control subjects, with a mean (SE) value of 4.2 (0.8) nmol/hour. Sulphation was significantly impaired (p less than 0.01) in 19 patients with active ulcerative colitis (0.6 (0.2) nmol/hour) and in 17 patients with ulcerative colitis in remission (1.1 (0.4) nmol/hour). Sulphation in eight patients with Crohn's colitis (4.3 (2.1) nmol/hour) was similar to that in control subjects. Impairment of the capacity of the mucosa to sulphate phenols in quiescent and active ulcerative colitis may pose a metabolic burden on colonic epithelial cells, which are continuously exposed to endogenous phenols from the colonic lumen.
蛋白质在人体结肠中经细菌发酵可产生大量酚类物质。在动物的结肠黏膜中，酚类物质主要通过与硫酸盐结合而失活。本研究的目的是证实人离体结肠细胞对酚类物质的硫酸化作用，并使用对乙酰氨基酚（扑热息痛）在直肠透析袋中评估炎症性肠病中的黏膜硫酸化作用。从切除的结肠标本（n = 7）中分离出的结肠细胞与对乙酰氨基酚孵育60分钟后，硫酸对乙酰氨基酚的平均（标准误）值为7.0（0.9）μmol/g干重，而对乙酰氨基酚葡萄糖醛酸苷的值几乎检测不到。所有对照受试者的直肠透析液中均检测到硫酸对乙酰氨基酚，平均（标准误）值为4.2（0.8）nmol/小时。19例活动期溃疡性结肠炎患者（0.6（0.2）nmol/小时）和17例缓解期溃疡性结肠炎患者（1.1（0.4）nmol/小时）的硫酸化作用明显受损（p < 0.01）。8例克罗恩病结肠炎患者（4.3（2.1）nmol/小时）的硫酸化作用与对照受试者相似。在静止期和活动期溃疡性结肠炎中，黏膜对酚类物质硫酸化能力的受损可能给持续暴露于来自结肠腔的内源性酚类物质的结肠上皮细胞带来代谢负担。
