Gibson P R, van de Pol E, Barratt P J, Doe W F
Department of Medicine and Clinical Science, John Curtin School of Medical Research, Australian National University, Woden Valley Hospital, Canberra.
Gut. 1988 Apr;29(4):516-21. doi: 10.1136/gut.29.4.516.
The leakiness of the cell membranes of colonic epithelial cells isolated by the collagenase/Dispase technique from normal or diseased colons was assessed in a 4 h 51Cr release assay. Cells from normal, adenoma bearing or cancer bearing colons showed 51Cr release of 8% or less in almost all of 46 cell populations tested. In contrast, cells from mucosa affected by ulcerative colitis [11.9 (4.3%) n = 23] or Crohn's disease [8.4 (2.7%) n = 18] released significantly more 51Cr than the non-inflamed groups. Values are expressed as mean (SD). Overall, release values were greater in ulcerative colitis than Crohn's disease (p less than 0.01). In Crohn's disease, cells obtained from histologically inflamed mucosa released significantly more 51Cr [9.7 (2.5%) n = 11] than those from non-inflamed mucosa [6.4 (1.5%) n = 7, p less than 0.02] whereas, in ulcerative colitis, abnormal release values were found in 8 of 13 cell populations isolated from mucosa showing no histological evidence of active disease. In five patients with distal ulcerative colitis, cells from mucosa not apparently involved demonstrated normal 51Cr release in four of five studies despite abnormal release from cells from involved mucosa suggesting that a diffuse abnormality of the colonic epithelial cell is not usually present. These data indicate that chronic mucosal inflammation per se is associated with abnormalities of the colonic epithelial cell but that, in ulcerative colitis, the abnormality remains in many patients with quiescent disease. Identification of the local factors responsible for such an abnormality may contribute to an understanding of the pathogenesis of ulcerative colitis.
通过胶原酶/中性蛋白酶技术从正常或患病结肠分离出的结肠上皮细胞的细胞膜通透性，在一项4小时的51铬释放试验中进行了评估。在几乎所有测试的46个细胞群体中，来自正常结肠、腺瘤性结肠或癌性结肠的细胞显示51铬释放率为8%或更低。相比之下，溃疡性结肠炎[11.9(4.3%)，n = 23]或克罗恩病[8.4(2.7%)，n = 18]受累黏膜的细胞释放的51铬明显多于未发炎组。数值以平均值(标准差)表示。总体而言，溃疡性结肠炎的释放值高于克罗恩病(p < 0.01)。在克罗恩病中，从组织学上发炎的黏膜获得的细胞释放的51铬[9.7(2.5%)，n = 11]明显多于未发炎黏膜的细胞[6.4(1.5%)，n = 7，p < 0.02]；而在溃疡性结肠炎中，从无活动病变组织学证据的黏膜分离出的13个细胞群体中有8个出现异常释放值。在5例远端溃疡性结肠炎患者中，尽管受累黏膜细胞释放异常，但在5项研究中有4项显示，来自未明显受累黏膜的细胞51铬释放正常，这表明结肠上皮细胞通常不存在弥漫性异常。这些数据表明，慢性黏膜炎症本身与结肠上皮细胞异常有关，但在溃疡性结肠炎中，许多病情静止的患者仍存在异常。确定导致这种异常的局部因素可能有助于理解溃疡性结肠炎的发病机制。
