Department of Haematology, Oncology and Clinical Immunology, Heinrich-Heine-University, Moorenstrasse 5, Düsseldorf, Germany.
Arch Physiol Biochem. 2009 Dec;115(5):286-97. doi: 10.3109/13813450903428086.
In the last decade proteomics has made great progress reaching throughput and comprehensiveness comparable to genomics technologies. Mass spectrometry plays a key role in proteomics and has become an indispensable method for molecular and cellular biology because many cellular changes in response to internal or external stimuli can only be detected at the proteome level. Furthermore, different from genomics which depends on the availability of DNA or RNA, proteomics is not restricted to cellular samples, but also allows the analysis of biological fluids like serum, plasma or urine. This article provides an overview of the recent developments in proteomics techniques useful for haematological research.
在过去的十年中，蛋白质组学取得了巨大的进展，其通量和全面性可与基因组学技术相媲美。质谱在蛋白质组学中起着关键作用，已成为分子和细胞生物学不可或缺的方法，因为许多细胞对内源或外源刺激的反应变化只能在蛋白质组水平上检测到。此外，与依赖 DNA 或 RNA 可用性的基因组学不同，蛋白质组学不仅限于细胞样本，还允许对生物体液（如血清、血浆或尿液）进行分析。本文概述了对血液学研究有用的蛋白质组学技术的最新进展。
