School of Health Sciences, Purdue University, West Lafayette, Indiana, USA.
Zebrafish. 2009 Dec;6(4):355-60. doi: 10.1089/zeb.2009.0600.
The zebrafish system has been established as a useful model for the study of carcinogenesis. The cytogenetic characterization of the genome is vital for furthering our understanding of the progression of the disease. Establishing a basic description of the zebrafish chromosomal karyotype and markers for each specific chromosome permitted the first cytogenetic characterization of the reference genome and the genome of cancer models. As the field of cancer cytogenetics is highly dependent on technology, each advance in technique and methodology has resulted in a corresponding wave of discoveries. We have witnessed great improvement in the resolution of the assays allowing for more detailed characterization of cytogenetic abnormalities, including the efficient and accurate identification of DNA copy number alterations of specific chromosomal regions. Herein, we will discuss major advancements in the field of cytogenetics, along with examples of how these technologies have been utilized in studies to characterize zebrafish cancer disease models. Finally, we will discuss the current state of the field and how microarray technology are being implemented to scan the whole genome at high resolution for DNA copy number alterations observed in various cancer types throughout the progression of the disease.
斑马鱼系统已被确立为研究致癌作用的有用模型。对基因组的细胞遗传学特征进行描述对于深入了解疾病的进展至关重要。建立斑马鱼染色体核型的基本描述和每个特定染色体的标记，使我们能够首次对参考基因组和癌症模型的基因组进行细胞遗传学特征描述。由于癌症细胞遗传学领域高度依赖技术，因此技术和方法学的每一次进步都带来了相应的发现浪潮。我们已经见证了检测分辨率的巨大提高，从而能够更详细地描述细胞遗传学异常，包括对特定染色体区域的 DNA 拷贝数改变进行高效和准确的鉴定。本文将讨论细胞遗传学领域的主要进展，并举例说明这些技术如何用于研究以描述斑马鱼癌症疾病模型。最后，我们将讨论该领域的现状以及如何实施微阵列技术，以高分辨率扫描整个基因组，以观察在疾病进展过程中各种癌症类型中观察到的 DNA 拷贝数改变。
