Oggé Giovanna, Romero Roberto, Chaiworapongsa Tinnakorn, Gervasi Maria Teresa, Pacora Percy, Erez Offer, Kusanovic Juan Pedro, Vaisbuch Edi, Mazaki-Tovi Shali, Gotsch Francesca, Mittal Pooja, Kim Yeon Mee, Hassan Sonia S
Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, Maryland, USA.
J Matern Fetal Neonatal Med. 2010 Jun;23(6):476-87. doi: 10.3109/14767050903216033.
Preeclampsia and pregnancies complicated by small-for-gestational age (SGA) neonates share several underlying mechanisms of disease. However, while an exaggerated systemic maternal inflammatory response is regarded as one of the hallmarks of the pathogenesis of preeclampsia, the presence of a similar systemic intra-vascular inflammation in mothers of SGA neonates without hypertension is controversial. The aim of this study was to determine phenotypic and metabolic changes in granulocytes and monocytes of women who develop preeclampsia and those who deliver an SGA neonate, compared to normal pregnant women.
This cross-sectional study included patients with a normal pregnancy (n = 33), preeclampsia (n = 33), and an SGA without preeclampsia (n = 33), matched for gestational age at blood sample collection. Granulocyte and monocyte phenotypes were determined by flow cytometry, using monoclonal antibodies against selective cluster of differentiation (CD) antigens. The panel of antibodies included the following: CD11b, CD14, CD16, CD18, CD49d, CD62L, CD64, CD66b, and HLA-DR. Intracellular reactive oxygen species (iROS) were assessed at the basal state and after stimulation (oxidative burst). Results were reported as mean channel brightness (MCB) or intensity of detected fluorescence. Analysis was conducted with non-parametric statistics. A p-value < 0.01 was considered statistically significant.
(1) Women who delivered an SGA neonate had a higher MCB of CD11b in granulocytes and monocytes than those with a normal pregnancy (p < 0.001 for both); (2) patients with preeclampsia had a lower median MCB of CD62L in granulocytes (p = 0.006) and a higher median basal iROS and oxidative burst in monocytes than women with an SGA neonate (p = 0.003 and p = 0.002, respectively).
Pregnancies complicated by the delivery of an SGA neonate are characterized by a higher activation of maternal peripheral leukocytes than in normal pregnancies, but lower than in pregnancies complicated by preeclampsia.
子痫前期和合并小于胎龄(SGA)新生儿的妊娠具有若干共同的潜在发病机制。然而,尽管母体全身性炎症反应过度被视为子痫前期发病机制的标志之一,但在无高血压的SGA新生儿母亲中是否存在类似的全身性血管内炎症仍存在争议。本研究的目的是确定与正常孕妇相比,发生子痫前期的妇女和分娩SGA新生儿的妇女的粒细胞和单核细胞的表型及代谢变化。
本横断面研究纳入了妊娠正常(n = 33)、子痫前期(n = 33)和无子痫前期的SGA(n = 33)患者,这些患者在采集血样时的孕周相匹配。使用针对选择性分化簇(CD)抗原的单克隆抗体,通过流式细胞术测定粒细胞和单核细胞表型。抗体组合包括:CD11b、CD14、CD16、CD18、CD49d、CD62L、CD64、CD66b和HLA - DR。在基础状态和刺激后(氧化爆发)评估细胞内活性氧(iROS)。结果以平均通道亮度(MCB)或检测到的荧光强度报告。采用非参数统计进行分析。p值<0.01被认为具有统计学意义。
(1)分娩SGA新生儿的妇女粒细胞和单核细胞中CD11b的MCB高于正常妊娠妇女(两者p均<0.001);(2)子痫前期患者粒细胞中CD62L的MCB中位数较低(p = 0.006),单核细胞中基础iROS和氧化爆发的中位数高于分娩SGA新生儿的妇女(分别为p = 0.003和p = 0.002)。
与正常妊娠相比,分娩SGA新生儿的妊娠的特征是母体外周白细胞的激活程度更高,但低于子痫前期妊娠。