Institute of Hydrobiology, the Chinese Academy of Sciences, Wuhan, P.R. China.
Anim Genet. 2010 Apr;41(2):191-8. doi: 10.1111/j.1365-2052.2009.01985.x. Epub 2009 Nov 16.
Common carp (Cyprinus carpio) is an important fish for aquaculture, but genomics of this species is still in its infancy. In this study, a linkage map of common carp based on Amplified Fragment Length Polymorphism (AFLP) and microsatellite (SSR) markers has been generated using gynogenetic haploids. Of 926 markers genotyped, 151 (149 AFLPs, two SSRs) were distorted and eliminated from the linkage analyses. A total of 699 AFLP and 20 microsatellite (SSR) markers were assigned to the map, which comprised 64 linkage groups and covered 5506.9 cM Kosambi, with an average interval distance of 7.66 cM Kosambi. The normality tests on interval map distances showed a non-normal marker distribution. Visual inspection of the map distance distribution histogram showed a cluster of interval map distances on the left side of the chart, which suggested the occurrence of AFLP marker clusters. On the other hand, the lack of an obvious cluster on the right side showed that there were a few big gaps which need more markers to bridge. The correlation analysis showed a highly significant relatedness between the length of linkage group and the number of markers, indicating that the AFLP markers in this map were randomly distributed among different linkage groups. This study is helpful for research into the common carp genome and for further studies of genetics and marker-assisted breeding in this species.
鲤鱼（Cyprinus carpio）是水产养殖中的重要鱼类，但该物种的基因组学仍处于起步阶段。本研究利用雌核发育单倍体构建了基于扩增片段长度多态性（AFLP）和微卫星（SSR）标记的鲤鱼连锁图谱。在 926 个基因型标记中，有 151 个（149 个 AFLP、2 个 SSR）发生偏分离，无法用于连锁分析。共分配到 699 个 AFLP 和 20 个 SSR 标记，该图谱包括 64 个连锁群，覆盖了 5506.9 cM Kosambi，平均标记间距离为 7.66 cM Kosambi。对连锁图谱距离的正态性检验表明，标记的分布是非正态的。图谱距离分布直方图的直观观察显示，图表左侧存在一组连锁图谱距离的聚类，这表明 AFLP 标记聚类的发生。另一方面，右侧没有明显的聚类表明存在一些较大的间隙，需要更多的标记来填补。相关性分析表明，连锁群的长度与标记数量之间存在高度显著的相关性，表明该图谱中的 AFLP 标记在不同的连锁群中随机分布。本研究有助于鲤鱼基因组的研究，也有助于该物种的遗传学和标记辅助育种的进一步研究。
