CIGENE - Centre of Integrative Genetics, As, Norway.
Anim Genet. 2010 Feb;41(1):48-54. doi: 10.1111/j.1365-2052.2009.01963.x. Epub 2009 Nov 16.
A better understanding of the genotype-phenotype correlation of Atlantic salmon is of key importance for a whole range of production, life history and conservation biology issues attached to this species. High-density linkage maps integrated with physical maps and covering the complete genome are needed to identify economically important genes and to study the genome architecture. Linkage maps of moderate density and a physical bacterial artificial chromosome (BAC) fingerprint map for the Atlantic salmon have already been generated. Here, we describe a strategy to combine the linkage mapping with the physical integration of newly identified single nucleotide polymorphisms (SNPs). We resequenced 284 BAC-ends by PCR in 14 individuals and detected 180 putative SNPs. After successful validation of 152 sequence variations, genotyping and genetic mapping were performed in eight salmon families comprising 376 individuals. Among these, 110 SNPs were positioned on a previously constructed linkage map containing SNPs derived from expressed sequence tag (EST) sequences. Tracing the SNP markers back to the BACs enabled the integration of the genetic and physical maps by assigning 73 BAC contigs to Atlantic salmon linkage groups.
更好地理解大西洋鲑的基因型-表型相关性对于与该物种相关的一系列生产、生活史和保护生物学问题至关重要。需要高密度的连锁图谱与物理图谱相结合，覆盖整个基因组，以鉴定具有经济重要性的基因，并研究基因组结构。已经生成了大西洋鲑的中等密度连锁图谱和物理细菌人工染色体（BAC）指纹图谱。在这里，我们描述了一种将连锁作图与新鉴定的单核苷酸多态性（SNP）的物理整合相结合的策略。我们通过 PCR 对 284 个 BAC 末端进行了重测序，在 14 个个体中检测到 180 个潜在的 SNP。在成功验证了 152 个序列变异后，在由 376 个个体组成的 8 个鲑鱼家族中进行了基因分型和遗传作图。其中，110 个 SNP 被定位在一个先前构建的包含来自表达序列标签（EST）序列的 SNP 的连锁图谱上。通过将 73 个 BAC 连续体分配给大西洋鲑的连锁群，追踪 SNP 标记回到 BAC 使遗传图谱和物理图谱得以整合。
