Laboratory of Gene Analysis, Department of Viral Oncology, Institute for Virus Research, Kyoto University, Kyoto, Japan.
Cancer Sci. 2010 Feb;101(2):536-42. doi: 10.1111/j.1349-7006.2009.01411.x. Epub 2009 Oct 22.
Human papillomaviruses (HPVs) infect the stratified epithelial organ. The infection induces benign tumors, which occasionally progress into malignant tumors. To elucidate the virus-induced tumorigenesis, an understanding of the lifecycle of HPV is crucial. In this report, we developed a new system for the analysis of the HPV lifecycle. The new system consists of a novel HPV replicon and an organotypic "raft" culture, by which the HPV-DNA is maintained stably in normal human keratinocytes for a long period and the viral vegetative replication is reproduced. This system will benefit biochemical and genetic studies on the lifecycle of HPV and tumorigenesis. This system is also valuable in screening for antiviral compounds. We confirmed its usefulness by evaluating the antivirus effect of cytokines.
人乳头瘤病毒（HPV）感染分层上皮器官。该感染会引发良性肿瘤，偶尔也会进展为恶性肿瘤。为了阐明病毒诱导的肿瘤发生，了解 HPV 的生命周期至关重要。在本报告中，我们开发了一种用于分析 HPV 生命周期的新系统。该新系统由新型 HPV 复制子和器官型“筏”培养物组成，通过该系统可使 HPV-DNA 在正常人类角质形成细胞中长期稳定维持，并且可再现病毒的营养复制。该系统将有利于 HPV 生命周期和肿瘤发生的生化和遗传研究。该系统在筛选抗病毒化合物方面也很有价值。我们通过评估细胞因子的抗病毒作用证实了该系统的有用性。
