Cheng J, Zhou L, Qin Y S, Wang Y, Xie H Y, Feng X W, Zheng S S
Department of Surgery, the First Affiliated Hospital, Zhejiang University, Zhejiang Province, People's Republic of China.
Transplant Proc. 2009 Nov;41(9):3893-7. doi: 10.1016/j.transproceed.2009.05.033.
Uptake of apoptotic cells by antigen-presenting cells (APC) may be involved in tolerance maintenance with an immunoregulatory role. The aim of this study was to evaluate the consequences of preoperative transfusion of donor apoptotic lymphocytes on survival of orthotopic liver transplantations (OLT). OLT was performed between Lewis (donor) and Brown Norway (BN recipient) inbred rats using a double-cuff technique. Apoptotic splenic lymphocytes induced by ultraviolet-C (UVC) irradiation were infused intravenously at 7 days before OLT. Changes in regulatory T cells in blood were determined using flow cytometry. UVC irradiated lymphocytes were sensitive and effective, as evidenced by increased peripheral blood CD4(+)CD25(+) T cells compared with recipients that received infusion of untreated donor lymphocytes or a control. Apoptotic lymphocyte transfusion prolonged hepatic allograft survival, with significantly lower histological stages of inflammation and cellular infiltration than in untreated allografts. Our results demonstrated that donor apoptotic cells promoted allograft acceptance and up-regulated CD4(+)CD25(+) regulatory T cells (Treg) in blood.
抗原呈递细胞（APC）对凋亡细胞的摄取可能参与具有免疫调节作用的耐受性维持。本研究的目的是评估术前输注供体凋亡淋巴细胞对原位肝移植（OLT）存活的影响。使用双袖套技术在Lewis（供体）和Brown Norway（BN受体）近交系大鼠之间进行OLT。在OLT前7天静脉内输注经紫外线-C（UVC）照射诱导的凋亡脾淋巴细胞。使用流式细胞术测定血液中调节性T细胞的变化。与接受未处理供体淋巴细胞输注或对照的受体相比，UVC照射的淋巴细胞敏感且有效，这可通过外周血CD4(+)CD25(+) T细胞增加来证明。凋亡淋巴细胞输注延长了肝同种异体移植的存活时间，其炎症和细胞浸润的组织学阶段明显低于未处理的同种异体移植。我们的结果表明，供体凋亡细胞促进了同种异体移植的接受，并上调了血液中的CD4(+)CD25(+)调节性T细胞（Treg）。
