Sun H, Lu X, Wu S, Sun W
Qilu Hospital, Shandong University, Jinan, Shandong Province, China.
Transplant Proc. 2009 Nov;41(9):3909-12. doi: 10.1016/j.transproceed.2009.06.190.
This study was designed to investigate the role of C-reactive protein (CRP), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) during allograft adventitial inflammation and arteriosclerosis. Lewis rats were used as donors and Wistar rats as recipients for the experimental groups, while both donors and recipients were Wistar rats in the control groups. The 36 experimental and 16 control allografts were divided into four groups (nine in each experimental and four in each control group): group A animals were euthanized at 1 week posttransplantation; groups B, C, and D animals were euthanized at 2, 3, and 4 weeks posttransplantation, respectively. The method of enzyme-linked immunosorbent assay (ELISA) was used to test serum levels of CRP, IL-6, and TNF-alpha. Immunohistological and pathological studies were performed to evaluate allograft adventitial inflammation, arteriosclerosis, and expression of proliferating cell nuclear antigen (PCNA). Allografts collected from group D demonstrated abundant infiltration of smooth muscle cells and collagenous fibers with inflammatory cells in the adventitia. ELISA demonstrated a higher levels of CRP, IL-6, and TNF-alpha in experimental than control groups. Additionally, PCNA increased over time in the adventitia. Our results suggested that the expressions of CRP, IL-6, and TNF-alpha play important roles during the development of allograft adventitial inflammation and arteriosclerosis.
本研究旨在探讨C反应蛋白（CRP）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）在同种异体移植物外膜炎症和动脉硬化过程中的作用。实验组以Lewis大鼠为供体，Wistar大鼠为受体，而对照组的供体和受体均为Wistar大鼠。36个实验性移植物和16个对照移植物被分为四组（每组实验性移植物9个，每组对照移植物4个）：A组动物在移植后1周处死；B、C和D组动物分别在移植后2、3和4周处死。采用酶联免疫吸附测定（ELISA）法检测血清中CRP、IL-6和TNF-α的水平。进行免疫组织学和病理学研究以评估同种异体移植物的外膜炎症、动脉硬化以及增殖细胞核抗原（PCNA）的表达。从D组收集的同种异体移植物显示外膜中有丰富的平滑肌细胞和胶原纤维浸润以及炎症细胞。ELISA显示实验组中CRP、IL-6和TNF-α的水平高于对照组。此外，外膜中的PCNA随时间增加。我们的结果表明，CRP、IL-6和TNF-α的表达在同种异体移植物外膜炎症和动脉硬化的发展过程中起重要作用。
