Box H/ACA 核糖核蛋白复合物：在转录后 RNA 修饰中 RNA 和蛋白质结构的相互作用。
The box H/ACA ribonucleoprotein complex: interplay of RNA and protein structures in post-transcriptional RNA modification.
机构信息
Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
出版信息
J Biol Chem. 2010 Jan 8;285(2):805-9. doi: 10.1074/jbc.R109.076893. Epub 2009 Nov 16.
Abstract
The box H/ACA ribonucleoproteins (RNPs) are protein-RNA complexes responsible for pseudouridylation, the most abundant post-transcriptional modification of cellular RNAs. Integrity of its box H/ACA domain is also essential for assembly and stability of the human telomerase RNP. The recent publication of the complete box H/ACA RNP structures combined with the previously reported structures of the protein and RNA components makes it possible to deduce the structural accommodation that accompanies assembly of the full particle. This analysis reveals how the protein components distort the RNA component of the RNP, enabling productive docking of the substrate RNA into the enzymatic active site.
摘要
盒 H/ACA 核糖核蛋白（RNP）是负责假尿嘧啶化的蛋白-RNA 复合物，假尿嘧啶化是细胞 RNA 中转录后修饰最丰富的一种。其盒 H/ACA 结构域的完整性对于人端粒酶 RNP 的组装和稳定性也是必不可少的。最近发表的完整盒 H/ACA RNP 结构，结合以前报道的蛋白质和 RNA 成分的结构，使得可以推断出伴随整个颗粒组装的结构适应性。该分析揭示了蛋白成分如何使 RNP 的 RNA 成分发生扭曲，从而使底物 RNA 有效地对接进入酶的活性部位。
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