Yao Wenlong, Qian Wei, Zhu Chang, Gui Lingli, Qiu Jin, Zhang Chuanhan
Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Neuroreport. 2010 Jan 6;21(1):39-44. doi: 10.1097/WNR.0b013e32833312fe.
The anaphase-promoting complex (APC) is a multisubunit E3 ubiquitin ligase that controls cell cycle transition in proliferating cells. Recent studies show that Cdh1-APC is active in postmitotic neurons, which regulates axonal growth and patterning, synaptic development and neuronal survival. However, the role of Cdh1-APC in neural stem cells differentiation remains unknown. Using quantitative reverse transcription-PCR, we observed that Cdh1 was expressed higher in neurons than in neural stem cells in vitro. Cdh1 was upregulated, whereas Id2 (one downstream substrate of Cdh1-APC) was downregulated when primary neural stem cells were induced to differentiate into neurons by all-trans retinoic acid. This observation suggests that Cdh1 is involved in the control of neural stem cells differentiated into neurons.
后期促进复合物（APC）是一种多亚基E3泛素连接酶，可控制增殖细胞中的细胞周期转换。最近的研究表明，Cdh1-APC在有丝分裂后的神经元中具有活性，可调节轴突生长和模式形成、突触发育以及神经元存活。然而，Cdh1-APC在神经干细胞分化中的作用仍然未知。通过定量逆转录PCR，我们观察到在体外培养时，神经元中Cdh1的表达高于神经干细胞。当原代神经干细胞被全反式维甲酸诱导分化为神经元时，Cdh1上调，而Id2（Cdh1-APC的一个下游底物）下调。这一观察结果表明，Cdh1参与了神经干细胞向神经元分化的调控。
