Zhang Xue-Bao, Zeng Yuan-Shan, Zhang Wei, Chen Ya-Yun, Zhang Wei, Xiong Yi, Chen Sui-Jun
Division of Neuroscience, Department of Histology and Embryology, Zhongshan Medical College, Sun Yat-Sen University, Guangzhou, Guangdong, China.
Biomed Environ Sci. 2006 Jun;19(3):219-24.
To investigate the synergistic effect of Schwann cells (SCs) and retinoic acid (RA) on differentiation and synaptogenesis of neural stem cells (NSCs) derived from hippocampus of neonatal rats.
The classical method for 2x2 factorial analysis experiment was used to assess synergistic action of SCs and RA. NSCs were treated with RA, SCs, and SCs + RA in DMEM/F12 with 0.5% fetal bovine serum for six days, respectively. Double immunofluorescent staining was used to detect the differentiation of NSCs including nestin, glial fibrillary acidic protein (GFAP) and Map2. The expression of PSD95 was used to demonstrate synaptogenesis.
After NSCs were treated with RA or SCs, the expression of nestin and GFAP was significantly decreased while the expression of Map2 and PSD95 was significantly increased in comparison with the control. Factorial ANOVA showed that interactions between SCs and RA could induce the expression of Map2 and PSD95.
SCs and RA could promote synergistically the neuronal differentiation and synaptogenesis of hippocampal neural stem cells in vitro while they decreased the astrocytes and nestin positive NSCs.
探讨雪旺细胞（SCs）和视黄酸（RA）对新生大鼠海马神经干细胞（NSCs）分化和突触形成的协同作用。
采用经典的2×2析因分析实验方法评估SCs和RA的协同作用。将NSCs分别在含0.5%胎牛血清的DMEM/F12培养基中用RA、SCs以及SCs + RA处理6天。采用双重免疫荧光染色检测NSCs的分化情况，包括巢蛋白、胶质纤维酸性蛋白（GFAP）和微管相关蛋白2（Map2）。通过检测突触后致密蛋白95（PSD95）的表达来证明突触形成。
与对照组相比,NSCs经RA或SCs处理后，巢蛋白和GFAP的表达显著降低，而Map2和PSD95的表达显著增加。析因方差分析表明，SCs和RA之间的相互作用可诱导Map2和PSD95的表达。
SCs和RA在体外可协同促进海马神经干细胞的神经元分化和突触形成，同时减少星形胶质细胞和巢蛋白阳性的NSCs。
