State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China, and Graduate School of the Chinese Academy of Sciences, Beijing 100039, China.
J Am Chem Soc. 2009 Oct 28;131(42):15082-3. doi: 10.1021/ja9051075.
A cation-driven allosteric G-quadruplex DNAzyme (PW17) was utilized to devise a conceptually new class of DNA logic gate based on cation-tuned ligand binding and release. K(+) favors the binding of hemin to parallel-stranded PW17, thereby promoting the DNAzyme activity, whereas Pb(2+) induces PW17 to undergo a parallel-to-antiparallel conformation transition and thus drives hemin to release from the G-quadruplex, deactivating the DNAzyme. Such a K(+)-Pb(2+) switched G-quadruplex, in fact, functions as a two-input INHIBIT logic gate. With the introduction of another input EDTA, this G-quadruplex can be further utilized to construct a reversibly operated IMPLICATION gate.
一种阳离子驱动的构象变构 G-四链体 DNA 酶 (PW17) 被用于设计一类基于阳离子调控配体结合与释放的新概念 DNA 逻辑门。K(+) 有利于血红素与平行链 PW17 的结合，从而促进 DNA 酶的活性，而 Pb(2+) 诱导 PW17 发生平行-反平行构象转变，从而驱使血红素从 G-四链体中释放，使 DNA 酶失活。实际上，这种 K(+)-Pb(2+) 切换的 G-四链体充当了一个双输入抑制逻辑门。通过引入另一个输入 EDTA，这个 G-四链体可以进一步用于构建一个可反复操作的蕴涵逻辑门。
