钾-铅切换的 G-四链体：一类新型 DNA 逻辑门。
Potassium-lead-switched G-quadruplexes: a new class of DNA logic gates.
机构信息
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China, and Graduate School of the Chinese Academy of Sciences, Beijing 100039, China.
出版信息
J Am Chem Soc. 2009 Oct 28;131(42):15082-3. doi: 10.1021/ja9051075.
A cation-driven allosteric G-quadruplex DNAzyme (PW17) was utilized to devise a conceptually new class of DNA logic gate based on cation-tuned ligand binding and release. K(+) favors the binding of hemin to parallel-stranded PW17, thereby promoting the DNAzyme activity, whereas Pb(2+) induces PW17 to undergo a parallel-to-antiparallel conformation transition and thus drives hemin to release from the G-quadruplex, deactivating the DNAzyme. Such a K(+)-Pb(2+) switched G-quadruplex, in fact, functions as a two-input INHIBIT logic gate. With the introduction of another input EDTA, this G-quadruplex can be further utilized to construct a reversibly operated IMPLICATION gate.
一种阳离子驱动的构象变构 G-四链体 DNA 酶 (PW17) 被用于设计一类基于阳离子调控配体结合与释放的新概念 DNA 逻辑门。K(+) 有利于血红素与平行链 PW17 的结合，从而促进 DNA 酶的活性，而 Pb(2+) 诱导 PW17 发生平行-反平行构象转变，从而驱使血红素从 G-四链体中释放，使 DNA 酶失活。实际上，这种 K(+)-Pb(2+) 切换的 G-四链体充当了一个双输入抑制逻辑门。通过引入另一个输入 EDTA，这个 G-四链体可以进一步用于构建一个可反复操作的蕴涵逻辑门。
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