Conger J D, Sage H J, Kawaguchi S, Corley R B
Department of Microbiology and Immunology, Duke University Medical Center, Durham, NC 27710.
J Immunol. 1991 Feb 15;146(4):1216-9.
We have analyzed the combining site diversity of murine antibodies reactive with bromelain-treated mouse RBC (BrMRBC) in B10.A mice. Although several VH and V kappa genes are used to generate antibodies of this specificity, two combinations account for more than 80% of the repertoire from the spleen: VH11/V kappa 9 and VH12/V kappa 4. Antibodies representing these two predominant groups were found to correspond to two previously reported distinct cross-reactive Id families accounting for most of the anti-BrMRBC reactivity in several strains of mice. mAb of the VH11/V kappa 9 type bound the haptens trimethyl-ammonium and phosphorylcholine much more avidly (approximately 1000 fold) than did the VH12/V kappa 4 type antibodies. Both of these haptens represent constituents of phosphatidylcholine, which BrMRBC-specific antibodies are reported to bind. Despite this differential reactivity, members of both the VH11/V kappa 9 and VH12/V kappa 4 antibody groups lysed BrMRBC with similar efficiencies. These data suggest that the two major classes of BrMRBC-specific antibodies have at least partially different specificities and imply that the events that lead to their high frequencies in the CD5+ repertoire may result from different selective forces.
我们分析了B10.A小鼠中与菠萝蛋白酶处理的小鼠红细胞（BrMRBC）反应的鼠抗体的结合位点多样性。尽管几种VH和Vκ基因用于产生具有这种特异性的抗体，但两种组合占脾脏抗体库的80%以上：VH11/Vκ9和VH12/Vκ4。发现代表这两个主要组的抗体对应于两个先前报道的不同的交叉反应性独特型家族，它们在几种小鼠品系中占大多数抗BrMRBC反应性。VH11/Vκ9型单克隆抗体比VH12/Vκ4型抗体更 avidly（约1000倍）地结合半抗原三甲基铵和磷酰胆碱。这两种半抗原都是磷脂酰胆碱的成分，据报道BrMRBC特异性抗体与之结合。尽管有这种不同的反应性，但VH11/Vκ9和VH12/Vκ4抗体组的成员以相似的效率裂解BrMRBC。这些数据表明，两类主要的BrMRBC特异性抗体至少部分具有不同的特异性，并暗示导致它们在CD5+抗体库中高频率出现的事件可能源于不同的选择力。
