B-1 derived anti-Thy-1 B cells in old aged mice develop lymphoma/leukemia with high expression of CD11b and Hamp2 that different from TCL1 transgenic mice.

Human old aged unmutated chronic lymphocytic leukemia U-CLL are the TCL1ZAP70CD5 B cells. Since CD5 makes the BCR signaling tolerance, ZAP70 increased in U-CLL not only TCL1 alone. In mice, TCL1 (TCL1A) is the negative from neonate to old aged, as TC. V8-12/V21-5 is the anti-thymocyte/Thy-1 autoreactive ATA B cell. When ATA μκTg generation in mice, ATA B cells are the neonate generated CD5 B cells in B-1, and in the middle age, CD5 can be down or continuously CD5, then, old aged CLL/lymphoma generation with increased CD11b in TCZAP70CD5 or TCZAP70CD5. In this old aged TCATA B microarray analysis showed most similar to human CLL and U-CLL, and TCZAP70CD5 showed certain higher present as U-CLL. Original neonate ATA B cells showed with several genes down or further increase in old aged tumor, and old aged T-betCD11c, CTNNB1, HMGB, CXCR4, DPP4 and decreased miR181b. These old aged increased genes and down miR181b are similar to human CLL. Also, in old age ATA B cell tumor, high CD38CD44, increased Ki67 AID, and decreased CD180 miR15O are similar to U-CLL. In this old aged ATA B, increased TLR7,9 and Wnt10b. TCTg generated with ATAμκTg mice occurred middle age tumor as TCZAP70CD5 or TCZAP70CD5, with high NF-kB1, TLR4,6 and Wnt5b,6 without increased CD11b. Since neonatal state to age with TCTg continuously, middle age CLL/lymphoma generation is not similar to old aged generated, however, some increased in TCZAP70 are similar to the old age TC ATA B tumor. Then, TC ATA B old age tumor showed some difference to human CLL. ATA B cells showed CD11bCD22, CD24 down, and hepcidin Hamp2 with iron down. This mouse V8-12 similar to human V2-5, and V2-5 showed several cancers with macrophages/neutrophils generated hepcidin iron or some showed hepcidin iron with tumor, and mouse V8-12 with different V19-17 generate MZ B cells strongly increased macrophage in old aged and generated intestine/colon tumor. Conclusion, neonate generated TCATA B1 cells in old aged tumor generation are CD11b in the leukemia CLL together with lymphoma cancer with hepcidin-related Hamp2 in B-1 cell generation to control iron.