Georgiana Dvorak Theobald Ophthalmic Pathology Laboratory, Department of Ophthalmology and Visual Sciences, Lions of Illinois Eye Research Institute, UIC Eye Center, University of Illinois at Chicago College of Medicine, Chicago, Illinois, U.S.A.
J Glaucoma. 1993 Summer;2(2):80-6.
We evaluated the effects of three specific glycosaminoglycan-degrading enzymes, chondroitinase ABC, testicular hyaluronidase, and heparinase, on the intraocular pressure (IOP) and the anterior chamber structures of bovine eyes. The anterior segment of freshly enucleated bovine eyes was perfused in a modified organ culture system. After each enzyme solution or its corresponding vehicle was introduced into the anterior chamber, the IOP was monitored for </=6 h. Compared to the control group, the chondroitinase ABC-treated group showed a significant IOP reduction after 1 h of perfusion. Testicular hyaluronidase and heparinase did not show any significant effect. Light microscopy revealed that chondroitinase ABC and testicular hyaluronidase induced detachment of the endothelial cells from trabecular beams in the uveal meshwork. Disruption of the tissues in the juxtacanalicular region and fragmentation of the endothelial cells facing the angular aqueous plexus were observed in the chondroitinase ABC- and heparinase-treated specimens. Electron microscopic study revealed detachment of trabecular meshwork cells from the beams and rupture of the inner wall of the aqueous plexus in all the enzyme-treated eyes. Cellular organelles and architectures were better preserved in chondroitinase ABC-treated eyes than those treated with hyaluronidase and heparinase. These results indicate that chondroitinase ABC has a pronounced effect on the IOP with relatively minor effect on the trabecular meshwork structures.
我们评估了三种特定糖胺聚糖降解酶（软骨素酶 ABC、睾丸透明质酸酶和肝素酶）对牛眼眼内压（IOP）和前房结构的影响。新鲜取出的牛眼前段在改良的器官培养系统中进行灌注。在将每种酶溶液或其相应的载体引入前房后，监测 IOP 时间为 </=6 小时。与对照组相比，软骨素酶 ABC 处理组在灌注 1 小时后表现出明显的 IOP 降低。睾丸透明质酸酶和肝素酶没有显示出任何显著的效果。光镜显示，软骨素酶 ABC 和睾丸透明质酸酶诱导了内皮细胞从葡萄膜网小梁束上的分离。在软骨素酶 ABC 和肝素酶处理的标本中观察到了在管周区组织的破坏和面对角状房水丛的内皮细胞的碎片。电子显微镜研究显示，在所有酶处理的眼中，小梁网细胞从梁上分离，房水丛的内壁破裂。在软骨素酶 ABC 处理的眼中，细胞细胞器和结构的保存优于透明质酸酶和肝素酶处理的眼睛。这些结果表明，软骨素酶 ABC 对 IOP 有明显的影响，对小梁网结构的影响相对较小。
