Department of Ophthalmology, University of California, San Diego, California, U.S.A.
J Glaucoma. 1994 Spring;3(1):70-8.
The ciliary muscle forms a major portion of the uveoscleral aqueous outflow pathway. To gain a better understanding of the extracellular matrix of the human ciliary muscle, human ciliary muscle cell cultures at various stages of confluency were immunocytochemically compared with sections of normal ciliary muscle for the presence of collagen types I, III, and IV, fibronectin, and laminin. A pre-embedding immunostaining protocol was employed to obtain good antigen conservation and histologically well-preserved tissue. In subconfluent cultured ciliary muscle cells, collagen types I, III, and IV, fibronectin, and laminin were found intracellularly in the endoplasmic reticulum or in granules surrounding the nucleus. In confluent cells, these matrix molecules each formed characteristic extracellular networks. In normal ciliary muscle, collagen type IV, fibronectin, and laminin were found in the basement membranes around the muscle fibers and blood vessels. Collagen types I and III and fibronectin were observed in the connective tissue among the muscle bundles. Collagen type I also formed a dense band of staining around blood vessels. The biosynthesis and turnover of these glycoproteins may have important roles both in the function of the nonconventional outflow pathway and in mediating the action of certain pharmacological agents.
睫状肌是葡萄膜巩膜房水流出途径的主要组成部分。为了更好地了解人睫状肌的细胞外基质，我们用免疫细胞化学的方法对处于不同汇合阶段的人睫状肌细胞培养物与正常睫状肌组织切片进行了比较，以观察Ⅰ、Ⅲ和Ⅳ型胶原、纤维连接蛋白和层粘连蛋白的存在情况。我们采用了一种包埋前免疫染色方案，以获得良好的抗原保存和组织学上保存完好的组织。在未汇合的培养睫状肌细胞中，Ⅰ、Ⅲ和Ⅳ型胶原、纤维连接蛋白和层粘连蛋白在内质网或围绕核的颗粒中呈细胞内存在。在汇合细胞中，这些基质分子各自形成了特征性的细胞外网络。在正常睫状肌中，Ⅳ型胶原、纤维连接蛋白和层粘连蛋白存在于肌纤维和血管周围的基底膜中。Ⅰ型和Ⅲ型胶原以及纤维连接蛋白存在于肌束之间的结缔组织中。Ⅰ型胶原还在血管周围形成了一条密集的染色带。这些糖蛋白的生物合成和周转可能在非传统流出途径的功能以及某些药理制剂作用的介导中具有重要作用。
