Tamm E, Rittig M, Lütjen-Drecoll E
Anatomisches Institut, Universität Erlangen-Nürnberg, Bundesrepublik Deutschland.
Fortschr Ophthalmol. 1990;87(6):623-9.
Topically applied prostaglandin F2 alpha (PGF2 alpha) has been shown to lower intraocular pressure in cynomolgus monkeys. In this study the morphological changes following topical treatment of seven cynomolgus monkeys with 4 micrograms PGF2 alpha isopropylester for 5-8 days were investigated and compared with the eyes of five normal animals. Quantitative light microscopical, ultrastructural and immunhistochemical methods were used. No cellular signs of inflammation were seen in any of the eyes. Slight edema in the most anterior part of the ciliary processes occurred in most eyes, but only in parts of the circumference. The most pronounced change was dilation of the intramuscular spaces within the ciliary muscle. No changes were observed in the extracellular matrix within the muscle bundles, which consist partly of type IV and VI collagen, laminin and fibronectin. In the connective tissue between the muscle bundles, loss of collagen type I and III fibrils was observed. Additionally, macrophages were found with phagolysosomes containing phagocytized collagen fibrils. We suggest that loss of extracellular material leads to a widening of the uveoscleral outflow pathways of ciliary muscle and thereby to a reduction in intraocular pressure.
局部应用前列腺素F2α（PGF2α）已被证明可降低食蟹猴的眼压。在本研究中，对7只食蟹猴局部应用4微克PGF2α异丙酯，持续5 - 8天，研究其形态学变化，并与5只正常动物的眼睛进行比较。采用了定量光学显微镜、超微结构和免疫组织化学方法。在任何一只眼睛中均未观察到炎症的细胞迹象。大多数眼睛的睫状体最前部出现轻微水肿，但仅在部分圆周范围内。最明显的变化是睫状肌内肌间隙扩张。在部分由IV型和VI型胶原、层粘连蛋白和纤连蛋白组成的肌束内细胞外基质中未观察到变化。在肌束之间的结缔组织中，观察到I型和III型胶原纤维的丢失。此外，发现巨噬细胞含有吞噬胶原纤维的吞噬溶酶体。我们认为细胞外物质的丢失导致睫状肌葡萄膜巩膜流出途径增宽，从而使眼压降低。
