Winkler F K, D'Arcy A, Blöcker H, Frank R, van Boom J H
European Molecular Biology Laboratory, Heidelberg, Germany.
J Mol Biol. 1991 Jan 20;217(2):235-8. doi: 10.1016/0022-2836(91)90536-f.
Complexes of the type II restriction endonuclease EcoRV with a variety of short, selfcomplementary deoxyoligonucleotides have been crystallized. The best crystals diffract to about 2.7 A resolution and consist of 1:1 complexes between endonuclease dimers and duplexes of the cognate decamer GGGATATCCC containing the hexameric RV recognition sequence GATATC. Crystals with the non-cognate DNA octamer duplexes CGAGCTCG and CGAATTCG diffract to 3.0 and 3.5 A resolution, respectively, and contain two DNA duplexes per enzyme dimer.
II型限制性内切酶EcoRV与多种短的、自我互补的脱氧寡核苷酸形成的复合物已被结晶。最佳晶体的衍射分辨率约为2.7埃，由内切酶二聚体与包含六聚体RV识别序列GATATC的同源十聚体GGGATATCCC的双链体之间的1:1复合物组成。与非同源DNA八聚体双链体CGAGCTCG和CGAATTCG形成的晶体，其衍射分辨率分别为3.0埃和3.5埃，每个酶二聚体包含两条DNA双链体。
